Decolorization of Diazo Dye Amido Black 10B by a Bacterium Kerstersia sp. Strain VKY1

Ramesh S. Masarbo; Pooja V. Reddy; Anand S. Nayak; T.B. Karegoudar

doi:10.37591/rrjomv.v8i3.446

Abstract

Amido Black 10B, a diazo dye having various industrial applications was decolorized by a bacterium Kerstersia sp. strain VKY1. The effect of various parameters such as dye concentration, pH, temperature and NaCl concentration was investigated. At 200 mg/l dye concentration, the strain decolorized 81% of the dye at temperature 35°C and pH 7.0 in 36 h of incubation. This strain decolorized the dye over a wide range of pH (5–11), temperature (25–55°C), and NaCl concentration (5–30 g/l). Further, the strain decolorized Amido Black 10B up to 600 mg/l in 48 h. The cell free extract of the strain VKY1 grown on Amido Black 10B exhibited the azoreductase activity of 0.7034 µM/min/mg protein. UV-Vis and HPLC investigation of the dye decolorized sample indicated the formation of breakdown products of azo bond. Keywords: Azo dye, toxic, decolorization, Amido Black 10B, Kerstersia sp., azoreductaseCite this ArticleMasarbo Ramesh S, Reddy Pooja V, Karegoudar TB et al. Decolorization of Diazo Dye Amido Black 10B by a Bacterium Kerstersia sp. Strain VKY1. Research & Reviews: A Journal of Microbiology and Virology. 2018; 8(3): 37–43p.