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Research and Reviews : A Journal of Life Sciences

Isolation and Characterization of Gelatinase Producing Halophilic Bacteria from the Sediments of Pichavaram Mangrove Forest, Tamil Nadu State, South India

  1. V. Balakrishnan
  2. M. Aarthi
  3. P. Eswaran
  4. M. Prasanna Rajeshkumar

Abstract

Microorganisms are the most important sources for production of enzyme especially bacteria which have the ability to synthesis gelatinase enzyme has been screened and isolated from Pichavaram mangrove forest soil. This enzyme is capable of degrading gelatin and collagen proteins. The organism was isolated from the mangrove sediments; the genomic DNA was extracted, amplified by 785F and 907R primers, sequenced and identified. Gelatinase producing organism was identified as Bacillus thuringiensis. This organism is also considered as a halotolerant (tolerates up to 14% NaCl concentration) and it could grow even at 0% (without) NaCl concentration. B.thuringiensis are able to produce gelatinase enzyme which degrades gelatin.Keywords: Amplification of rDNA, DNA sequencing, DNA, gelatinase, halophilicCite this ArticleM. Aarthi, P. Eswaran, M. Prasanna Rajeshkumar, V. Balakrishnan. Isolation and Characterization of Gelatinase Producing Halophilic Bacteria from the Sediments of Pichavaram Mangrove Forest, Tamil Nadu State, South India. Research & Reviews: A Journal of Life Sciences. 2019; 9(3): 63–70p.

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