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Research & Reviews: A Journal of Drug Formulation, Development and Production Original Research

A Research on Formulation and Evaluation of Clove Gel for Treatment of Scabies

  1. Bhagyashri N. Patil Department of Pharmaceutics, Poojya Sane Guruji Vidya Prasarak Mandal’s College of Pharmacy, Shahada
  2. Bhupendra A. Patil Department of Pharmaceutics, Poojya Sane Guruji Vidya Prasarak Mandal’s College of Pharmacy, Shahada
  3. Bhagvati V. Patil Department of Pharmaceutics, Poojya Sane Guruji Vidya Prasarak Mandal’s College of Pharmacy, Shahada
  4. Atharv K. Patil Department of Pharmaceutics, Poojya Sane Guruji Vidya Prasarak Mandal’s College of Pharmacy, Shahada
  5. Amruta N. Patil Department of Pharmaceutical Chemistry, Poojya Sane Guruji Vidya Prasarak Mandal’s College of Pharmacy, Shahada
  6. Sunil P. Pawar Poojya Sane Guruji Vidya Prasarak Mandal’s College of Pharmacy

Abstract

Scabies remains a pervasive global health issue, with a need for more effective topical treatments. This research focuses on the formulation and evaluation of a clove oil-based gel containing eugenol, the primary bioactive component, for the treatment of scabies. Eugenol’s well-documented antimicrobial, anti-inflammatory, and ant parasitic activities make it an ideal candidate for targeting Sarcoptes scabiei, the mite responsible for scabies. The formulation was prepared by incorporating clove oil and eugenol into a gel base with suitable excipients such as gelling agents, preservatives, and stabilizers. Key parameters like physical appearance, pH, homogeneity, spread ability, and extrudability were systematically evaluated to optimize the gel’s therapeutic properties and user acceptability. Eugenol, as the active pharmaceutical ingredient, was used to ensure targeted therapeutic efficacy, while excipients were selected to enhance the gel's stability and application.

Keywords

References (34)

  1. Swe PM, Christian LD, Lu HC, Sriprakash KS, Fischer K. Complement inhibition by Sarcoptes scabiei protects Streptococcus pyogenes - An in vitro study to unravel the molecular mechanisms behind the poorly understood predilection of S. pyogenes to infect mite-induced skin lesions. PLOS Neglected Tropical Diseases. 2017;11(3):e0005437. doi:10.1371/journal.pntd.0005437
  2. Micali G, Lacarrubba F, Verzì AE, Chosidow O, Schwartz RA. Scabies: Advances in Noninvasive Diagnosis. PLOS Neglected Tropical Diseases. 2016;10(6):e0004691. doi:10.1371/journal.pntd.0004691
  3. Kandi V. Laboratory Diagnosis of Scabies Using a Simple Saline Mount: A Clinical Microbiologist's Report. Cureus. 2017. doi:10.7759/cureus.1102
  4. Stamm LV, Strowd LC. Ignoring the “Itch”: The Global Health Problem of Scabies. The American Journal of Tropical Medicine and Hygiene. 2017;97(6):1647-1649. doi:10.4269/ajtmh.17-0242
  5. Vasanwala F, Ong C, Aw C, How C. Management of scabies. Singapore Medical Journal. 2019;60(6):281-285. doi:10.11622/smedj.2019058
  6. Anderson KL, Strowd LC. Epidemiology, Diagnosis, and Treatment of Scabies in a Dermatology Office. The Journal of the American Board of Family Medicine. 2017;30(1):78-84. doi:10.3122/jabfm.2017.01.160190
  7. Dressler C, Rosumeck S, Sunderkötter C, Werner RN, Nast A. The Treatment of Scabies. Deutsches Ärzteblatt international. 2016. doi:10.3238/arztebl.2016.0757
  8. Álvarez-Soria MA, Herrero-Beaumont G, Moreno-Rubio J, Calvo E, Santillana J, Egido J, et al. Long-term NSAID treatment directly decreases COX-2 and mPGES-1 production in the articular cartilage of patients with osteoarthritis. Osteoarthritis and Cartilage. 2008;16(12):1484-1493. doi:10.1016/j.joca.2008.04.022
  9. Li L, Rossoni G, Sparatore A, Lee LC, Del Soldato P, Moore PK. Anti-inflammatory and gastrointestinal effects of a novel diclofenac derivative. Free Radical Biology and Medicine. 2007;42(5):706-719. doi:10.1016/j.freeradbiomed.2006.12.011
  10. Cevc G, Gebauer D, Stieber J, Schätzlein A, Blume G. Ultraflexible vesicles, Transfersomes, have an extremely low pore penetration resistance and transport therapeutic amounts of insulin across the intact mammalian skin. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1998;1368(2):201-215. doi:10.1016/s0005-2736(97)00177-6
  11. Trommer H, Neubert RHH. Overcoming the Stratum Corneum: The Modulation of Skin Penetration. Skin Pharmacology and Physiology. 2006;19(2):106-121. doi:10.1159/000091978
  12. Karande P, Mitragotri S. Enhancement of transdermal drug delivery via synergistic action of chemicals. Biochimica et Biophysica Acta (BBA) - Biomembranes. 2009;1788(11):2362-2373. doi:10.1016/j.bbamem.2009.08.015
  13. Milind P, Deepa K. Clove: A champion spice. Int J Res Ayur Pharm. 2011;2(1):47–54.
  14. Hussain S, Rahman R, Mushtaq A, Zerey-Belaskri AE. Clove: A review of a precious spice with multiple uses. Int J Chem Bio Sci.
  15. Cock IE, Chessman M. Plants of the genus Syzygium (Myrtaceae): A review on ethnobotany, medicinal properties, and phytochemistry. In: Goyal MR, Ayeleso A, editors. Bioactive Compounds of Medicinal Plants. USA: Apple Academic Press; 2018.
  16. Hu Q, Zhou M, wei S. Progress on the Antimicrobial Activity Research of Clove Oil and Eugenol in the Food Antisepsis Field. Journal of Food Science. 2018;83(6):1476-1483. doi:10.1111/1750-3841.14180
  17. Yadav M, Yadav P, Sahu S, Yadav V, Gupta SN. Review of literature on clove. Int J Creative Res Thought. 2021;9(1):1883–1888.
  18. Nassar M, Gaara AH, El-Ghorab AH, Farrag ARH. Chemical constituents of clove (Syzygium aromaticum, Fam. Myrtaceae) and their antioxidant activity. J Chem Nat Compd. 2007;35(3).
  19. Bhowmik D, Kumar KS, Yadav A, Srivastava S, Paswan S, Dutta AS. Recent trends in Indian traditional herbs Syzygium aromaticum and its health benefits. J Pharmacogn Phytochem. 2012;1(1):13–22.
  20. Jirovetz L, Buchbauer G, Stoilova I, Stoyanova A, Krastanov A, Schmidt E. Chemical Composition and Antioxidant Properties of Clove Leaf Essential Oil. Journal of Agricultural and Food Chemistry. 2006;54(17):6303-6307. doi:10.1021/jf060608c
  21. Ho SH, Cheng LPL, Sim KY, Tan HTW. Potential of cloves (Syzygium aromaticum (L.) Merr. and Perry as a grain protectant against Tribolium castaneum (Herbst) and Sitophilus zeamais Motsch. Postharvest Biology and Technology. 1994;4(1-2):179-183. doi:10.1016/0925-5214(94)90019-1
  22. Kim EH, Kim HK, Ahn YJ. Acaricidal Activity of Clove Bud Oil Compounds againstDermatophagoides farinaeandDermatophagoides pteronyssinus(Acari: Pyroglyphidae). Journal of Agricultural and Food Chemistry. 2003;51(4):885-889. doi:10.1021/jf0208278
  23. Sarrami N, Pemberton MN, Thornhill MH, Theaker ED. Adverse reactions associated with the use of eugenol in dentistry. British Dental Journal. 2002;193(5):257-259. doi:10.1038/sj.bdj.4801539
  24. Batiha GES, Beshbishy AM, Tayebwa DS, Shaheen HM, Yokoyama N, Igarashi I. Inhibitory effects of Syzygium aromaticum and Camellia sinensis methanolic extracts on the growth of Babesia and Theileria parasites. Ticks and Tick-borne Diseases. 2019;10(5):949-958. doi:10.1016/j.ttbdis.2019.04.016
  25. Martínez-Herrera A, Pozos-Guillén A, Ruiz-Rodríguez S, Garrocho-Rangel A, Vértiz-Hernández A, Escobar-García DM. Effect of 4-Allyl-1-hydroxy-2-methoxybenzene (Eugenol) on Inflammatory and Apoptosis Processes in Dental Pulp Fibroblasts. Mediators of Inflammation. 2016;2016:1-7. doi:10.1155/2016/9371403
  26. Sethi A. Synthesis of eugenol. In: Systematic Laboratory Experiments in Organic Chemistry. New Age Publications.
  27. Pasay C, Mounsey K, Stevenson G, Davis R, Arlian L, Morgan M, et al. Acaricidal Activity of Eugenol Based Compounds against Scabies Mites. PLoS ONE. 2010;5(8):e12079. doi:10.1371/journal.pone.0012079
  28. Safitri FI, Nawangsari D, Febrina D. Overview: Application of Carbopol 940 in Gel. Proceedings of the International Conference on Health and Medical Sciences (AHMS 2020). 2021. doi:10.2991/ahsr.k.210127.018
  29. National Center for Biotechnology Information. PubChem compound summary for CID 1030, propylene glycol [Internet]. 2024 [Accessed from April 2024]. Available from: https://pubchem.ncbi.nlm.nih.gov/compound/Propylene-Glycol.
  30. Rathod H, Mehta D. A review on pharmaceutical gel. Acta Scient Int J Pharm Sci. 2015;1(1):33–47.
  31. Patil S, Gadade D, Rathi PB. Design, development, and evaluation of herbal gel for treatment of psoriasis. JIPBS. 2015;2(1):72–87.
  32. Misal G, Dixit G, Gulkarni V. Formulation and examination of herbal gel. Indian J Nat Prod Resour. 2012;3(4):501–505.
  33. Hess L, Tater K. Dermatologic diseases. In: Quesenberry KE, Carpenter JW, editors. Ferrets, Rabbits, and Rodents. 3rd ed. W. B. Saunders; 2012. pp. 232–244.
  34. Abou-Elghar GE, El-Sayed AM, Abdel-Aziz MF. Acaricidal effect of eugenol against Sarcoptes scabiei. J Parasitol Res. 2013;2013:981370.
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