By using amino acid residues the peptides can be synthesized using either solid phase peptide synthesis or solution phase peptide synthesis. In this research article we preferred solution phase peptide synthesis because the formed intermediate unnecessary products can be easily isolated and purified. On through literature survey we found that the solution phase synthesis of tripeptides employing HATU/HOBt as coupling agent by using microwave irradiation[720 watts] has been mentioning in this article. The coupling reaction completes within 30-45 seconds. We used totally 5-steps for the Tri-peptides synthesis and each step is detailed in the articleFinally, NMR and mass spectroscopic methods were used to characterize the synthesized tripeptide derivatives.
