Experimental Studies and in silico Analysis on Quarum Sensing and System Simulation in Pseudomonas aeruginosa

Anamika Rani Kesri; D.S.V.G.K. Kaladhar

doi:10.37591/rrjocb.v7i1.209

Abstract

Pseudomonas aeruginosa is the most common pathogenic bacterium present in human microbial flora, biofilms, soil and water associated and support self microbial communications and survilance. The present study shows estimated protein concentration present in two days grown in Mullar hinton broth was resulted around 32.5 mg/ml. The studies in protein interaction on X-ray film with aqueous fruit and vegetable extracts showed good results with apple, straw berry, cucumber, grape, orrange, papaya and cabbage more effectively and least interaction was showed in spinach, red spinach and menthi. The quantification studies showed band similar to standard (Albumin) at 67 kDa. Several proteins like LuxQ, LuxU, albumin and LuxP were involved in quorum sensing as per the previous studies conducted by KEGG database. The proteins were submitted to string database for understanding system simulation and the network constructed shows involvement of other proteins like pspF, rhoN, cheA, Hmp etc. Ligand-receptor docking results for the selected autoinducers of QS with receptors showed good activity of HMP protein with Ridaforolimus showing energy value of –134.9 kcal/mol. The results showed that autoinducer like Ridaforolimus plays key role in the mechanism of quorum sensing in P. aeruginosa.Keywords: Quorum sensing, system simulation, P. AeruginosaCite this Article Anamika Rani Kesri, DSVGK Kaladhar. Experimental Studies and in silico Analysis on Quarum Sensing and System Simulation in Pseudomonas aeruginosa. Research & Reviews: Journal of Computational Biology. 2018; 7(1): 1–8p.