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Research and Reviews : A Journal of Biotechnology Original Research

Application of Priestia Megaterium in the Bioremediation of Petroleum Hydrocarbon Contaminated Environments

  1. Rathi C R Department of Biotechnology, Mar Augusthinose College Ramapuram
  2. Thara Pradeep Department of Biotechnology, Mar Augusthinose College Ramapuram
  3. Prem Jose Vazhacharickal Student, Department of Agricultural Economics, University of Agricultural Economics
  4. Sajeshkumar N K Department of Biotechnology, Mar Augusthinose College Ramapuram

Abstract

Petroleum pollutants pose a serious and persistent threat to environmental ecosystems, particularly affecting soil and water quality. Effective and sustainable remediation strategies are crucial to mitigate their impact and safeguard environmental health. In this context, bioremediation—using microorganisms to degrade harmful contaminant —emerges as a promising and eco-friendly approach. The present study explores the biodegradation capabilities of the bacterium Priestia megaterium, a species previously recognized for its ability to break down hydrocarbons. The primary objective of this research is to isolate and characterize Priestia megaterium from soil samples obtained from areas in close proximity to a petrol pump, a site typically subjected to high levels of petroleum contamination. The study aims to evaluate the bacterium’s efficiency in degrading petroleum-based pollutants and investigate its applicability in bioremediation efforts. The research methodology involves the isolation of P. megaterium from petroleum-contaminated soil, followed by a comprehensive analysis through both biochemical assays and molecular characterization techniques. Further, the study assesses the hydrocarbon degradation potential of the bacterium under controlled conditions. This investigation aspires to contribute to the development of sustainable, low-cost, and environmentally friendly bioremediation technologies. By validating the efficacy of Priestia megaterium in degrading petroleum hydrocarbons, the findings are expected to support its application in field-scale bioremediation programs. Ultimately, the study aims to inform future strategies and enhance the efficiency of microbial-based clean-up of petroleum-contaminated sites.

Keywords

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