Predictive Modeling and Optimization of Citric Acid Bioproduction Using Ipomoea batatas Peel (Sweet Potato)

Uku, P. E.; Ekperi, N. I

Abstract

In this research, the Box–Behnken design was adopted and applied to the process and the total of 30 experimental runs were generated for the liquefaction steps considered for the purpose of citric acid bioproduction using Ipomoea batatas peel (sweet potato) which was very helpful during the investigatory process of this research. The liquefaction step adopted were very sensitive as gross investigations to ascertain their usability was made and proven worthy before being adopted as part of the research methodology which includes ANOVA test, the ANOVA test showed the quadratic model obtained to be significant (p<0.0001). For the liquefaction step, the R2 was 98.92% while the adjusted R2 was found to be over 96% and the entire p-value coefficients were <0.0001. However (X1X2, X1X3, X1X4) respectively were not less than 0.0001. This is a clear indication that the adopted model is suitable and buyable to represent the actual relationship between the given variable. The square root value obtained from research work shows a very efficient consistency between the observed values and the predicted values. To determine their reliability plots and values, a computer application software called MATLAB and Microsoft Excel was used to plot a 3D graph of the effect of temperature against time and to determine the deferential behaviors of both the predicted parameters and the actual values for RSM. In all, both the models and all the methods adopted for this process as listed and demonstrated in the methodology were considered perfect and reliable for citric acid bioproduction using Ipomoea batatas peel (sweet potato).

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