protease enzyme
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BIOCHEMICAL AND KINETICS STUDIES ON PROTEASE ENZYME ISOLATED FROM AYURVEDIC HERBS ALONG WITH THEIR APPLICATION STUDIES
Abstract: The objective of the present work was to conduct comprehensive biochemical and kinetic analysis of a protease enzyme extracted from two Ayurvedic herbs, namely Shankhpushpi (Convolvulus prostratus) and Mulethi (Glycyrrhiza glabra). The primary focus was to characterize various biochemical aspects of the enzyme, encompassing substrate specificity, optimal pH and temperature conditions, stability, and activation energy. Furthermore, crucial kinetic parameters such as Michaelis-Menten constant (Km) and maximum reaction velocity (Vmax) were …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 13, Issue 3, 2023 · pp. 21–37 Read article
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Isolation and Characterization of Protease Producing Organism Bacillus Species from Slaughter House Waste
Abstract: The purpose of this investigation was to isolate protease producing bacteria from slaughter house waste collected in local area of Kaliakkavilai. The Bacillus sp. was identified by several biochemical and physiological tests. The effect of different production parameters such as pH, temperature, metal ions concentration and substrate concentration for protease production by the isolated bacterial strain were studied. The enzyme production was assayed in submerged fermentation with optimized parameters to …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 3, 2013 · pp. 8–14 Read article