nucleic acid amplification
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Prevalence of HIV and TB Co-infection: Mumbai Scenario
Abstract: HIV, being the most powerful risk factor, has made the reappearance and progression of tuberculosis easier. The partnership of HIV and TB is a major health problem in both developed and developing countries. The present study was undertaken in Mumbai to study the prevalence of HIV and TB coinfection in patients who visited the tertiary care centers in the city. Both HIV and TB were diagnosed using nucleic acid amplification …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 3, Issue 2, 2013 · pp. 26–32 Read article
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Evaluation of Nucleic Acid Amplification Method in Diagnosing Enteric Fever in Children
Abstract: The comparison of three detection methods (Widal test, Typhi dot and Nested PCR) for diagnosis of typhoid fever was done in laboratory where gold standard was either Widal positive or Typhi dot positive or both positive. A nested polymerase chain reaction (PCR) specific for Salmonella enteric serovar typhi was used for the detection of the pathogen in blood samples. Total 52 patients with clinical suspicion of typhoid fever were recruited …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 1, 2013 · pp. 9–15 Read article