Non-homologous end joining

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• Published Subscription 28 Apr 2014

  Designer Zinc Finger Nuclease: A Versatile Tool for Genome Editing in Animals

  By Kiran Lakkireddy, Sudhakar Podha, Krishna Satya A, Kasturi K, Sambasiva Rao KRS

  Abstract: In this review, is provided an update on the most popular approaches, including zinc finger nuclease (ZFN)-based gene editing by homologous recombination (HR), gene knockout, non-homologous end joining (NHEJ), and gene therapy in mouse. Zinc finger nucleases (ZFNs) are synthetic restriction enzymes evolved by fusing zinc finger that facilitate genome editing by creating a double-stranded break in DNA at a user-specified location. ZFNs are new tools that promise to radically …

  Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 24–30 Read article →