MON810

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• Published Subscription 7 Jan 2013

  Semi-quantitative Event Specific Detection of Bt Maize (Event MON 810) using Real-Time PCR with SYBR Green Ι Chemistry

  By Dharmendra Mishra, Rashmi Kaushal, Prithviraj Khodake

  Abstract: The objective is this work is to do semi-quantitative analysis for Bt maize (event MON 810) using conventional Polymerase Chain Reaction (PCR) and Real Time PCR (SYBR green chemistry) from the fresh maize samples chosen arbitrarily from various locations. The protocol for extraction of good quality genomic Deoxyribonucleic acid (DNA) was standardized without the use of Liquid nitrogen. The DNA used for further studies were obtained by using standardized protocol. …

  Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 3, 2012 Read article →