Fc engineering

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• Published Subscription Review Article 5 May 2026

  Integrative Structural-Functional Genomics of Fc and Fab: Precision Models for Monoclonal Antibody Stability and Anti-Aggregation Engineering

  By Tapaswi Ram Khajuria, Prof (Dr.) Atul Khajuria, KIRAN KUMARI

  Abstract: Monoclonal antibodies (mAbs) represent the cornerstone of biotherapeutics, yet aggregation propensity compromises up to 50% of candidates during development, driven by Fab hypervariability and Fc vulnerabilities.(1,2) This review integrates functional genomics from OAS (4B+ sequences)(5) and structural databases (SAbDab: 10K+ structures)(6) with machine learning models achieving R=0.97 for SAP prediction.(11) We dissect biophysical mechanisms, benchmark predictive tools (DeepSP, ESM2), and engineering strategies (YTE, FW mutations) that enhance Tm by 5-10°C …

  Published in International Journal of Molecular Biotechnological Research · Vol. 4, Issue 1, 2026 Read article →

• Published Subscription Review Article 29 Apr 2026

  Metagenome-Informed Engineering of Monoclonal Antibodies: Mining Microbial Immunoglobulin-Like Domains for Ultra-Stable Therapeutics

  By Atul Khajuria, Ashish Kumar

  Abstract: Metagenome-informed engineering represents an emerging strategy for improving the stability and functionality of monoclonal antibodies (mAbs) by leveraging the vast diversity of microbial immunoglobulin-like (Ig-like) domains (10,24). These domains, derived from uncultured bacteria and bacteriophages, exhibit exceptional thermostability, protease resistance, and structural resilience due to adaptation to extreme environments (29,30). This review highlights advances in metagenomic mining, structural characterization, and protein engineering that enable the integration of microbial Ig-like scaffolds …

  Published in Research and Reviews : A Journal of Biotechnology · Vol. 16, Issue 1, 2026 Read article →