Biochemical analysis
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BIOCHEMICAL AND KINETICS STUDIES ON PROTEASE ENZYME ISOLATED FROM AYURVEDIC HERBS ALONG WITH THEIR APPLICATION STUDIES
Abstract: The objective of the present work was to conduct comprehensive biochemical and kinetic analysis of a protease enzyme extracted from two Ayurvedic herbs, namely Shankhpushpi (Convolvulus prostratus) and Mulethi (Glycyrrhiza glabra). The primary focus was to characterize various biochemical aspects of the enzyme, encompassing substrate specificity, optimal pH and temperature conditions, stability, and activation energy. Furthermore, crucial kinetic parameters such as Michaelis-Menten constant (Km) and maximum reaction velocity (Vmax) were …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 13, Issue 3, 2023 · pp. 21–37 Read article
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Comparative Responses of Some Fuel Wood Trees at Early Seedling Stage
Abstract: Seedling of leguminous fuel wood trees (Bauhinia variegata, Delonix regia, Peltophorum pterocarpum, Samanea saman, Saraca indica) were analyzed at different developmental stages for biochemical constituents potentially related to fast-growing habit in order to identify some markers. Protein content of leaves and stem tissue was higher in Bauhinia variegata, particularly at later stages. Total amino acid level, however, did not show much difference between growing species. Total carbohydrate content (both soluble …
Published in Research & Reviews : Journal of Botany · Vol. 9, Issue 2, 2020 · pp. 5–13 Read article