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2 articles for “semi-quantitative PCR.”
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A Comparative Study on the Expression of Various Housekeeping Genes in the Organs of Different Mice Strains
Abstract: The expression of housekeeping genes significantly varies between organs and between strains. Therefore, it is highly required to find out the housekeeping gene that can be the most useful and reliable one, where inter-organ comparative studies on gene expression need to be performed. Keeping this issue in consideration, a comparative study of different housekeeping genes using organs of four different strains of mice was performed. Different organs, i.e., liver, kidney, …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 5, Issue 3, 2015 · pp. 1–10 Read article
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Semi-quantitative Event Specific Detection of Bt Maize (Event MON 810) using Real-Time PCR with SYBR Green Ι Chemistry
Abstract: The objective is this work is to do semi-quantitative analysis for Bt maize (event MON 810) using conventional Polymerase Chain Reaction (PCR) and Real Time PCR (SYBR green chemistry) from the fresh maize samples chosen arbitrarily from various locations. The protocol for extraction of good quality genomic Deoxyribonucleic acid (DNA) was standardized without the use of Liquid nitrogen. The DNA used for further studies were obtained by using standardized protocol. …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 3, 2012 Read article