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6 articles for “knockout gene”
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CRISPR/CAS9 Technology and its Application in Food
Abstract: Advances in the editing technology of genome or genes and also availability for the genome sequence for several crops has developed possibility for the researcher to precisely target the desired phenotype. Such therapy is possible due to CRISPR editing technology, which involves various cloning methods. After proving this concept in certain plants or crops which were used for the demonstration, thus targeting and improving the specific working of CRISPR/CAS9 technology. …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 8, Issue 2, 2021 · pp. 38–42 Read article
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Designer Zinc Finger Nuclease: A Versatile Tool for Genome Editing in Animals
Abstract: In this review, is provided an update on the most popular approaches, including zinc finger nuclease (ZFN)-based gene editing by homologous recombination (HR), gene knockout, non-homologous end joining (NHEJ), and gene therapy in mouse. Zinc finger nucleases (ZFNs) are synthetic restriction enzymes evolved by fusing zinc finger that facilitate genome editing by creating a double-stranded break in DNA at a user-specified location. ZFNs are new tools that promise to radically …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 24–30 Read article
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Role of Crispr-Cas9 in Plants
Abstract: The innovative CRISPR Cas9 system has emerged as a game changing genetic engineering tool in years offering levels of precision, efficiency and flexibility in editing the genomes of various organisms, including plants. This summary aims to provide an examination of the role of CRISPR Cas9, in modifying plant genomes highlighting its benefits, drawbacks and possible uses. Thanks to its versatility and user-friendly nature CRISPR Cas9 technology has expedited studies in …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 14, Issue 3, 2024 · pp. 1–10 Read article
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Role of Gastric Receptors in Gastrointestinal Disorder
Abstract: The established and potential roles of cholecystokinin1 and cholecystokinin2 receptors in gastrointestinal and metabolic diseases are analyzed, along with findings from human studies involving agonists and antagonists. While there is considerable evidence implicating cholecystokinin1R in various diseases such as pancreatic disorders, motility disorders, tumor growth, satiety regulation, and cholecystokinin-deficient states, its specific role in these conditions remains ambiguous. Conversely, the role of cholecystokinin2R in physiological (e.g., atrophic gastritis) and pathological …
Published in International Journal of Biomedical Innovations and Engineering · Vol. 2, Issue 2, 2024 · pp. 37–49 Read article
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Simulation and Optimization of Syngas Generation Process (GTL Stage I) using ASPEN Hysys
Abstract: The process of gas to liquid (GTL) conversion has been divided into three sections (section I: natural gas treatment and syngas production; section II: is Fischer Tropsch Synthesis sections (Main Reactors) and Stage III: Upgrading and treatment section).This work is mainly for Stage I. whereby Natural gas dehydration unit is simulated using Tri-ethylene Glycol to dehydrate the natural gas which includes (Free Water Knockout Drum (FWKD), Absorber and Distillation). Sweetening …
Published in Journal of Petroleum Engineering & Technology · Vol. 10, Issue 2, 2020 · pp. 48–67 Read article
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CRISPR-Based Enhancement of Secondary Metabolites in Artemisia annua: Focus on Artemisinin Biosynthesis
Abstract: Medicinal plants are natural biofactories producing secondary metabolites with significant therapeutic value, including terpenoids, alkaloids, and phenolics. Among these, artemisinin, a sesquiterpene lactone from Artemisia annua L. is the cornerstone of global antimalarial therapy along with additional anticancer, antiviral, and antimicrobial activities. However, artemisinin content in the native plants is inherently low (typically 0.01–0.8% dry weight), restricting affordable and consistent supply. Conventional approaches to increase production include classical breeding, singlegene …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 16, Issue 2, 2026 · pp. 23–35 Read article