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4 articles for “broth dilution”
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Antibacterial and Antifungal Activities of Mirabilis jalapa of Indian Origin
Abstract: The in vitro antimicrobial activity of Mirabilis jalapa leaf extract was studied against selected pathogenic and non-pathogenic microorganisms Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae, Penicillium chrysogenum, Auriparus flaviceps and Trichoderma viridae by using agar diffusion and broth dilution methods. Leaves were extracted using solvents petroleum ether, chloroform and methanol. Among the solvents tested, petroleum ether and chloroform extracts showed good antimicrobial activity against the selected pathogens. The growth inhibitors were …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 2, 2012 · pp. 1–10 Read article
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Improvised Rapid Antibiotic Sensitivity Test by Disc Dispersion Method
Abstract: Multidrug resistance is the one of vital issue globally, there are several causes for multidrug resistance, like improper utilization of antibiotics and selection of suitable antibiotics are the one of the predominant reasons for multidrug resistance gaining. To enable physician to select the suitable antibiotics, appropriate antibiotic sensitivity shall be performed in shortest time period. A method has been developed to get the antibiotic sensitivity test in 2–3 hours for …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 44–57 Read article
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Green Synthesis of Selenium Nano-metalloid from the extract of Cabbage (Brassica oleracea var. capitata) leaf and its Antimicrobial Activities
Abstract: Green synthesis specifies the creation of nano-size particles (NPs) by a biological process using the reducing properties of metabolites in the plant extracts. The present work reports the use of cabbage (Brassica oleracea var. capitata.) leaf extract as a reducing and stabilizing agent to synthesize selenium nanoparticles (SeNPs) via the green route of synthesis. The as-prepared SeNPs were analyzed by UV–vis spectroscopy, FTIR, DLS, SEM, and EDX. Nano-crystalline SeNPs has …
Published in Journal of Nanoscience, NanoEngineering & Applications · Vol. 9, Issue 1, 2019 · pp. 12–20 Read article
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Detection of Listeria monocytogenes in Milk and Ice Cream by Polymerase Chain Reaction
Abstract: Abstract The present study was undertaken to standardize PCR assay for detection of L. monocytogenes and Listeriolysin O from livestock foods and compare its efficacy with conventional cultural methods. A set of primer derived from iap gene and other set derived from hlyA gene were used for detection of L. monocytogenes and Listeriolysin O in the PCR assay. The PCR assay was initially standardized by optimizing the concentration of the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 24–35 Read article