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12 articles for “V(D)J recombination”
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Integrative Structural-Functional Genomics of Fc and Fab: Precision Models for Monoclonal Antibody Stability and Anti-Aggregation Engineering
Abstract: Monoclonal antibodies (mAbs) represent the cornerstone of biotherapeutics, yet aggregation propensity compromises up to 50% of candidates during development, driven by Fab hypervariability and Fc vulnerabilities.(1,2) This review integrates functional genomics from OAS (4B+ sequences)(5) and structural databases (SAbDab: 10K+ structures)(6) with machine learning models achieving R=0.97 for SAP prediction.(11) We dissect biophysical mechanisms, benchmark predictive tools (DeepSP, ESM2), and engineering strategies (YTE, FW mutations) that enhance Tm by 5-10°C …
Published in International Journal of Molecular Biotechnological Research · Vol. 4, Issue 1, 2026 Read article
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A Note on Vaccine Design Using Bioinformatics Methods and Applications
Abstract: Vaccines prevent infectious diseases caused by viruses and bacteria saving millions of lives.It consists of certain agents that resemble disease causative agentsand stimulates our immune system to recognize and fight foreign antigens. Immune function or an immune response is the fundamental basis of the vaccine's mechanism. Vaccines are pharmaceutical derivatives that provide an affordable way to prevent diseases.Various bioinformatics approaches can be incorporated into vaccine design studies and development. Reverse …
Published in International Journal of Bioinformatics and Computational Biology · Vol. 1, Issue 1, 2023 · pp. 1–9 Read article
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Investigation of Electro-optical Characteristic and Structure Optimization of PMMA: Bepq2/BCP/Tpbi Blue Highly Efficient OLED Device
Abstract: In this Investigation, the energy level structure of the Spiro-MeOTAD/PMMA: Bepq2/BCP/Tpbi/LiF OLED has been investigated to explain the method of electron-hole charge carrier pair recombination and the light generation. The Maxwell-Boltzmann statistics model was applied to optimize the structure to reduce the band gap between conductive layers. We found the simulated results J–V, J-Illumination flux, and Current Efficiency (CE) characteristics when we applied forward bias. Inconclusive results determined the threshold …
Published in Journal of Polymer & Composites · Vol. 11, Issue 5, 2023 · pp. 56–68 Read article
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Design and Analysis of Quantum Dot Solar Cell Using SCAPS-1D Software
Abstract: Compared to conventional solar cells, quantum dot solar cells (QDSCs) have drawn a lot of attention due to their hybrid structure, low production cost, and greater power conversion efficiency. Nevertheless, carrier recombination in the quasi-neutral region (QNR) continues to limit their performance. The electron transport layer (ETL) and hole transport layer (HTL) are two of the layers that are most important in determining the overall efficiency of the device. The …
Published in Journal of Alternate Energy Sources & Technologies · Vol. 17, Issue 1, 2026 · pp. 15–20 Read article
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CRISPR, Recombinant DNA, and LAMP-Based Platforms for Monkeypox: Emerging Tools for Diagnosis and Therapeutic Development
Abstract: Monkeypox, a zoonotic viral infection caused by the Monkeypox virus of the Orthopoxvirus genus, has recurred as a worldwide public health concern after recent outbreaks outside of its classical endemic areas in Central and West Africa. The resurgence has demanded a thorough reassessment of its epidemiology, mode of transmission, and clinical presentation. In light of these challenges, recombinant DNA technology (rDNA) has emerged as a candidate for developing vaccines, diagnostics, …
Published in International Journal of Virus Studies · Vol. 3, Issue 1, 2026 · pp. 7–28 Read article
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Construction and Development of pSB111 Bar Plasmid Vector for Activation Tagging
Abstract: To get desired traits and to know the precise expression patterns of genes in the plant, Agrobacterium tumefaciens has been widely employed in the generation of transgenic plants using plasmid vectors. Many methods are come forward to establish the gene expression of unknown genes but in these techniques gene alteration isthe main drawback orsometimesit islethal to organisms also, to overcome these problems activator tag method developed to know the specific …
Published in Research & Reviews : Journal of Botany Read article
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A Decade of Viral Evolution (2015–2025): Emergence, Mutations, and Implications for Global Health
Abstract: Over the past decade (2015-2025), viral evolution has profoundly reshaped the global health landscape, challenging healthcare systems, diagnostic tools, therapeutic strategies, and vaccine efficacy. This review comprehensively analyzes the evolutionary trajectories of major human and zoonotic viruses, with a focus on pandemic-prone, drug-resistant, and emerging pathogens. Viral evolution is driven by diverse molecular mechanisms including high mutation rates, recombination, reassortment, host immune pressure, and antiviral interventions, leading to genetic diversification, …
Published in International Journal of Vaccines · Vol. 2, Issue 2, 2025 · pp. 31–40 Read article
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Recombinant Human EGF in Wound Healing: 3D Computational Insights
Abstract: A Growth Factor represents a naturally-occurring compound with the remarkable ability to incite cellular growth, proliferation, and differentiation. Typically manifesting as either a protein or a steroid hormone, these factors wield significant influence over an array of cellular activities. Their pivotal role lies in the regulation of diverse cellular processes, often serving as catalysts for cell specialization and maturation, a characteristic that distinguishes them from one another. For instance, bone …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 11, Issue 2, 2024 · pp. 6–10 Read article
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ZnO Nanostructure Shape Effects in Photocatalysis of Methylene Blue
Abstract: Nowadays, dyes are used in the food industry, textile industry, paper industry, hair coloring, light-harvesting arrays, photoelectrochemical cells, etc. Most of the dyes are nonbiodegradable, toxic, carcinogenic, and consist of organic compounds. These dye wastages are discharged into water bodies and the environment causing serious hazards to the marine ecosystem as well as the humans extensively. To treat toxic dyes traditional methods such as adsorption, activated carbon, ultrafiltration, and reverse …
Published in Journal of Polymer & Composites · Vol. 13, Issue 1, 2025 · pp. 722–727 Read article
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Ebola Vaccination: From Outbreak Control to Global Preparedness
Abstract: Ebola virus disease (EVD) is one of the most dreadful and lethal viral infections, continuously generating threatening impacts on the health of the global population, especially in low-resource environments. This review provides an overview of Ebola vaccines, focusing on virology, vaccine development, clinical trials, regulatory approval, deployment plans, and ethical issues. Recombinant viral vector platforms have seen the development of successful vaccines, with the most prominent example being rVSV-ZEBOV, which …
Published in International Journal of Vaccines · Vol. 3, Issue 1, 2026 · pp. 27–37 Read article
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Exploring the Genetic and Environmental Factors Contributing to Ovarian Cancer in Women in Mumbai, India
Abstract: Background: Ovarian cancer incidence in Mumbai has risen by 30% over the past decade, with an age-standardized rate of 9.1 per 100,000 women, contrasting stable trends in Western nations. This study investigates the interplay of genetic and environmental factors driving this disparity in Mumbai’s diverse population. Methods: A hospital-based case-control study was conducted at a hospital, enrolling 200 epithelial ovarian cancer cases (aged 30–70 years) and 400 age-matched controls. Germline …
Published in Research and Reviews: Journal of Oncology and Hematology · Vol. 14, Issue 3, 2025 · pp. 12–17 Read article
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An AAV Vector for Inducible Gene Expression Preferentially in Muscles
Abstract: Adeno associated viral (AAV) vectors has been used widely in gene therapy and efforts have been made to improve their utility by adding genetic elements that would enable targeting transgene expression to particular cells or tissues of interest and permitting on/off regulation of expression. In this study, we designed a recombinant AAV9 variant PHP.eB vector with muscle creatine kinase (Mck)-derived enhancers, a synthetic muscle-expression promoter, in combination with a third-generation …
Published in International Journal of Genetic Modifications and Recombinations · Vol. 1, Issue 2, 2023 · pp. 46–61 Read article