Search
5 articles for “Mutant Strains”
-
Biochemical Characterization of Mutant Strains and Cloning of Lactate Dehydrogenase Gene from Staphylococcus aureus
Abstract: An infection is the detrimental colonization of a host organism by a foreign species. About 15 million (>25%) of 57 million annual deaths worldwide are estimated to be related directly to infectious diseases. The burden of morbidity (ill health) and mortality associated with infectious diseases falls most heavily on people in developing countries and particularly on infants and children diseases. Infectious diseases are the second leading cause of death; the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 7, Issue 1, 2017 · pp. 15–25 Read article
-
Production of Phosphatase by Phosphate Solubilizing Improved Fungal Strains
Abstract: Phosphate solubilizing micro-organisms were isolated from rhizosphere soils of rice fields around Mannachanalur area, Tiruchirappali district of Tamilnadu. The isolated fungal cultures were identified as Aspergillus niger, Aspergillus fumigatus and Penicillium sp. The efficacy of Phosphatase activity of fungal strains was carried out. The fungal strains were subjected to random mutational studies such as UV and chemical mutation. The Phosphatase assay was carried out after random mutagenesis using wild and …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 2, Issue 1, 2012 · pp. 39–52 Read article
-
Partial Characterization of Crude Lipase from Mutant Soil Fungal Isolate
Abstract: Lipases are cluster of enzymes which cleaves oils and fats. Characterization properties oflipases have been studied in the present investigation because they play an imperative role inseveral industrial applications. The present lipase obtained from soil fungal strain named asEMS5%-60min exhibited optimum activity at pH 7.0 (24.90±0.25 IU/ml/min) and 37ºC (20.28±0.51IU/ml/min). In pH stability profile, the enzyme was found stable in alkaline pH range withhighest activity (13.08±0.07 IU/ml/min) at pH 9.0. …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 79–89 Read article
-
The Ames Test in Twenty-first Century
Abstract: Since the 19th century, industrial development has increased exposure to mutagens and possible carcinogens. In order to avoid these health risks, several techniques have been developed to recognize genotoxins. In the 1970s, Dr. Bruce Ames and colleagues, studying the histidine operon of Salmonella typhimurium, observed that histidine (−) auxotroph mutants were reverted to histidine (+) upon exposure to a mutagen. The researchers further observed that known carcinogens were mutagenic to …
Published in Research and Reviews: A Journal of Toxicology · Vol. 2, Issue 1, 2012 · pp. 23–37 Read article
-
Growth Related Production of Poly-ß-Hydroxybutyrate by Azotobacter chroococcum Soil Isolate/Mutant using N, P, and Cane Molasses
Abstract: Soil isolate Azotobacter chroococcum ABR 51 produced a large amount of Poly-ß-Hydroxybutyrate (PHB) during growth in a chemically defined medium using cane molasses as carbon source. Nutritional requirements and cultural conditions for the optimal production of PHB by this strain under laboratory conditions were determined using inorganic, complex organic nitrogen sources, phosphate, and cane molasses. Among conventional carbon sources, (2% w/v) sucrose was found to be the preferred carbohydrate source …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 1, Issue 2, 2011 · pp. 14–22 Read article