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196 articles for “Microbiological”
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Detection of Hepatitis B Virus Coinfection among HIV Infected Patients in District Bharatpur, Rajasthan
Abstract: Aim: To screen for the coinfection of hepatitis B virus (HBV) in human immunodeficiency virus (HIV) infected patients. Methods: Hundred consecutive HIV infected patients were screened for HBV (HBsAg) using commercially available ELISA kit. Results: HBV coinfection was detected in 5/100 (5%) HIV infected patients. Male gender predominance was observed (80%), (four males and one female) and the median age was 37 years (95%CI ± 3.6) (range 20–55 years). Coinfection …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 2, 2018 · pp. 11–13 Read article
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Comparison of Nested PCR and Real Time PCR of Herpes Simplex Virus Infection in Encephalitis Cases
Abstract: Introduction: Herpes simplex virus (HSV) causes a wide spectrum of clinical manifestations in the central nervous system (CNS). Presently, the development of effective antiviral therapy has resulted in an increased need for the rapid and sensitive diagnosis of HSV infections. Aims: To evaluate the usefulness of real-time PCR for diagnosing HSV encephalitis (HSE), we prospectively compared results of real-time PCR and nested PCR, using clinical samples. Methods and Material: viral …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 34–36 Read article
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Isolation and Characterization of Rhamnolipid from Pseudomonas aeruginosa: Assessment of Biofilm Disruption Potential
Abstract: Biosurfactants are microbial metabolites bearing typical amphiphilic structure of a surfactant. This study is aimed at isolation and characterization of Rhamnolipid (RL) biosurfactant from Pseudomonas aeruginosa MTCC 2488. Characterization of biosurfactant was assessed by oil displacement test, foaming test, thin layer chromatography (TLC) and antimicrobial activity for the same was evaluated against planktonic/sessile microbes including Staphylococcus aureus, Bacillus subtilis and Candida albicans. Keywords: Biosurfactant, rhamnolipid, biofilm, Pseudomonas aeruginosaCite this ArticleKaur …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 2, 2018 · pp. 26–33 Read article
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Evaluation of Antibiofilm Potential of Alginate Encapsulated Selenium Nanoparticles
Abstract: Biofilm interfere in antibiotic therapy, undergo gene transformation to become highly virulent, develop quorum sensing and protect bacteria from host immunity. To combat these biofilms the encapsulation method of nanoparticles formation using PEG can be used as they are better alternative to chemical and physical methods. In the present study, the antibiofilm and antioxidant properties of the selenium nanoparticles produced by using probiotics have been evaluated. Transmission electron microscopy (TEM) …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 2, 2018 · pp. 16–21 Read article
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Exploring the Potentials of Quercetin and Kaempferol combinations along with Regular Antibiotics for the Effective Management of Methicillin-resistant Staphylococcus aureus (MRSA)
Abstract: The methicillin-resistant S. aureus (MRSA) is a bacterium that offers the challenge to numerous antibiotics which leads to a variety of problems, ranging from skin infections and sepsis to pneumonia. The natural products based management somewhat opened hope towards the betterment of the present era where the combination with currently available medicines has been studied clinically. Antibiotics (cefixime, ceftriaxone, erythromycin, ciprofloxacin, cephradine, methicillin, ampicillin, levofloxacin, and amoxicillin) and flavonoids (quercetin, …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 6–9 Read article
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Partial Characterization of Crude Lipase from Mutant Soil Fungal Isolate
Abstract: Lipases are cluster of enzymes which cleaves oils and fats. Characterization properties oflipases have been studied in the present investigation because they play an imperative role inseveral industrial applications. The present lipase obtained from soil fungal strain named asEMS5%-60min exhibited optimum activity at pH 7.0 (24.90±0.25 IU/ml/min) and 37ºC (20.28±0.51IU/ml/min). In pH stability profile, the enzyme was found stable in alkaline pH range withhighest activity (13.08±0.07 IU/ml/min) at pH 9.0. …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 79–89 Read article
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Fungal Synthesis of Silver Nanoparticles by an Indigenous Species of Fusarium, Its Antibacterial Activity and Efficacy as Antibiotic Enhancer
Abstract: The investigations on green combination strategies and on its potential applications for the humanity are on incline for more than 10 decades in the field of bioscience. The research on silver nanoparticles by the use of microorganisms is very much popular these days as it is very easily available source for their synthesis. In the present work, an indigenous Fusarium species isolated from soil sample has been used for the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 10–18 Read article
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Colorimetric Redox Indicator assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis in South Gujarat region
Abstract: Tuberculosis (TB) has affected humans since antiquity. The burden of TB particularly with multi-drug-resistance (MDR) is increasing worldwide and has become a major public health concern. This study aims to perform Colorimetric Redox Indicator (CRI) assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis in South Gujarat region. Total 234 samples were collected from South Gujarat region and were subjected for microscopy examination, culturing on Lowenstein-Jensen (LJ) medium and drug susceptibility …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 65–70 Read article
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The Predatory Bacteria, Bdellovibrio and Like Organisms (BALOs): An Emerging Savior for Human Welfare
Abstract: Bdellovibrio and like organisms (BALOs) are capable of killing other Gram negative pathogenic and non-pathogenic bacteria dwelling in a diverse environmental niches such as soil, freshwater and ocean. The predatory nature of BALOs can be a viable alternative to conventional use of antibiotics to treat ocular, periodontal, respiratory and fish pathogen infections since the incessant use develops resistance against them. Unique property such as potential to survive in ecologically hazardous …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 19–33 Read article
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Production of High Yield Amylase and Protease by Aspergillus oryzae using Solid-State Fermentation
Abstract: Aspergillus oryzae strains were isolated from different sources and its ability to produce industrial scale amylase and protease using solid-state fermentation was carried in the present study. Different fermentation substrates such as rice bran and wheat bran were evaluated for maximum enzyme yield. Enzyme production was maximum on rice bran and it was achieved with optimized fermentation parameter such as temperature 30°C, pH 4–6, moisture content 60%, inoculum size 0.2 …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 71–78 Read article
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Screening of Cold-Active Lipase from Psychotropic Fungi
Abstract: Lipase is a class of enzymes that catalyze as, hydrolysis of long-chain tri-glycoside and comprise the large significant group of bio-catalysts for biotechnological applications. Currently, the cold-active lipases (CLPS) are the enzyme of choice for pharmacists, organic chemists, biochemical, biophysicists and engineering processes, bio-technologists and micro-biologists as well as biochemists. The production of CLPs is mostly existed from psychrophilic microorganisms. Psychrophiles and psychrotrophs extracellular colds-active enzymes at low temperature (cold) …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 1–5 Read article
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Diagnostic Modalities Used for Fungal Isolates Confirmed in Clinically Suspected Cases of Otomycosis: A Screening Study
Abstract: Otomycosis is one of the most attending ailed in ENT practice. Fungal infection of external auditory canal is called otomycosis. Discomfort or pain, itching, ear blockage and ear discharge are symptoms of otomycosis. Ear discharge is seen in the cases of bacterial otitis externa (Furuncle or diffuse otitis externa), otomycosis, acute otitis media, chronic otitis media, etc. So to confirm diagnosis of otomycosis, microbiology test of ear discharge is needed. …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 58–64 Read article
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Promising Antimicrobial Compounds from an Endophytic Fungus Penicillium daleae EF 4 Isolated from the Seaweed Enteromorpha flexuosa Linn.
Abstract: AbstractThe present aim of the study is to isolate the endophytic fungi from various seaweeds and to screen their bioactivity, to analyze the β-lactamase inactivator and to evaluate the interactions between the crude extract of Endophytic fungi Penicillium daleae EF 4 and two antibiotics (penicillin G and tetracycline), against some antibiotic resistant bacterial strains. Seaweeds collected from various places of Bay of Bengal coast was subjected to Endophytic fungal isolation. …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 26–39 Read article
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Decolorization of Diazo Dye Amido Black 10B by a Bacterium Kerstersia sp. Strain VKY1
Abstract: Amido Black 10B, a diazo dye having various industrial applications was decolorized by a bacterium Kerstersia sp. strain VKY1. The effect of various parameters such as dye concentration, pH, temperature and NaCl concentration was investigated. At 200 mg/l dye concentration, the strain decolorized 81% of the dye at temperature 35°C and pH 7.0 in 36 h of incubation. This strain decolorized the dye over a wide range of pH (5–11), …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 37–43 Read article
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Screening and Characterisation of Antibacterial Actinomycetes Isolated From Earthworm
Abstract: The actinomycetes are considered to be the most potent source for the production of secondary metabolites, antibiotics and other bioactive compounds. The present study dealt with the isolation of the actinomycetes from the earthworm, Eisenia fetida. The actinomycetes were identified using slide culture technique and various biochemical tests. The isolates (S1 and S2) showed activity against Gram positive and Gram negative bacteria in primary screening by perpendicular streak method. In …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 77–84 Read article
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Characterization of Laccase Enzyme from Dye Degrading Bacterial Strains Isolated from Natural Sources
Abstract: The continuous growth in globalization and industrialization has increased the environmental pollution and one such major contaminant is various types of synthetic dyes. Microbial decolorization and degradation of these dyes through secretion of various extracellular enzymes is gaining importance owing to their eco-friendly nature. One such enzyme is laccase, a multicopper oxidase enzyme which has the ability to oxidize both phenolic and non-phenolic compounds through reduction of molecular oxygen to …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 65–76 Read article
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Improvised Rapid Antibiotic Sensitivity Test by Disc Dispersion Method
Abstract: Multidrug resistance is the one of vital issue globally, there are several causes for multidrug resistance, like improper utilization of antibiotics and selection of suitable antibiotics are the one of the predominant reasons for multidrug resistance gaining. To enable physician to select the suitable antibiotics, appropriate antibiotic sensitivity shall be performed in shortest time period. A method has been developed to get the antibiotic sensitivity test in 2–3 hours for …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 44–57 Read article
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Taxonomic Assessment of Thermostable Amylase Producer from the Unkeshwar Hot Spring, Nanded
Abstract: Total 42 thermophiles were isolated from the Unkeshwar hot spring of Maharashtra, India. Of these 23 thermostable amylase producers were identified as Bacillus alvei APP1, Bacillus brevis APP2, Bacillus sterothermophilus APP3, Bacillus subtilis APP4, Bacillus farraginis APP5, Bacillus firmus APP6, Bacillus licheniformis APP7, Bacillus megaterium APP8, Lysinibacillus sphaericus APP10, Paenibacillus alvei APP11, Bacillus simplex APP12, Staphylococcus cohnii APP15, Streptococcus thermophilus APP16, Bacillus cereus APP18, Bacillus atrophaeus APP19, Bacillus lentus APP24, …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 113–126 Read article
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Production, Optimization and Purification of Laccase Produced by Pleurotus ostreatus MH591763
Abstract: The optimization of production medium for laccase produced by Pleurotus ostreatus (Gene Bank Accession number MH591763) to reduce the cost of laccase production in large scale is the basic research in industrial field. In this study, the laccase culture medium was optimized for growth parameters such as incubation period, pH, temperature, carbon and nitrogen source on fungal growth and enzyme production. Purification and characterization of the enzyme was done by …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 56–64 Read article
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Microbial Contaminants in Commercial Beverages: Health Concerns and Suggestive Preservative Methods
Abstract: AbstractFruit juices are the important source of vitamins, minerals and antioxidants, essential for the growth and development of every human. However, due to the presence of various nutritive components, it provides optimal conditions for the growth of food microorganisms. Various pathogenic and non-pathogenic bacteria, fungus, yeasts and parasites are found to be associated with fruit juices. Many of these microbes generally enters at the time of packaging and cause various …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 1–10 Read article