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25 articles for “PCR amplification”
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Multiplex PCR Assay for Rapid Detection of Different Strains of Escherichia coli Associated with Fish and Fishery Products
Abstract: A multiplex PCR (MPCR) assay was developed for the detection of Escherichia coli using selected primers designated on the species- and strains-specific genes such as hlyA, LT1 and phoA. The internal regions amplified were having a product size of 165, 275, and 903 bp, respectively. This MPCR assay had a sensitivity of detecting four cells of E. coli within 10 h in artificially contaminated shrimp tissue homogenate. The MPCR assay …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 4, Issue 3, 2014 · pp. 1–9 Read article
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Molecular Diagnosis of Rifampicin Antibiotic Resistance in Mycobacterium Avium Subspecies Paratuberculosis Isolated from Sheep Clinical Samples
Abstract: The present study was conducted to identify parts of the rpoB gene in MAP of Indian sheep isolates. The rpoB gene (Beta sub unit of RNA polymerase) is involved in conferring the rifampicin antibiotic resistance in MAP bacteria. The resistance is due to the presence of mutations in the 81 bp variable region of the rpoB gene including C1367T and T1442C mutations identified by several workers. The study was further …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 12, Issue 3, 2022 · pp. 35–56 Read article
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Transgenic Expression of Stress Tolerance Gene in Sugarcane (Saccharum officinarum L.)
Abstract: AbstractAgrobacterium tumefaciens LBA4404 mediated transformation of sugarcane variety 31 with two plasmids of pB1121 constructs, one containing PsCBL gene and the other containing PsCIPK gene was studied for stress tolerance capability induction. Calli of the variety Isd 31 were infected with both plasmid containing strains using three different infection times of 60, 90 and 120 min in acetosyringone infection medium. In GUS (β-glucuronidase) assay, randomly selected calli from 120 min …
Published in Research & Reviews : Journal of Agricultural Science and Technology · Vol. 6, Issue 2, 2017 · pp. 32–39 Read article
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Estimation of Genetic Diversity in Some Species of Meliaceae Family through RAPD Technique
Abstract: Random amplified polymorphic DNA (RAPD) analysis has proved to be useful in determining genetic relationship and variation in many plant species, result of RAPD analysis can be used to determine genetic distances and relatedness within or among species, which can then be utilized in construction of phytogenetic. RAPD analysis could also provide useful insights into the phylogenetic relationship. RAPD is a polymerase chain reaction (PCR), which is based on dominant …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 5, Issue 3, 2015 · pp. 11–17 Read article
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Molecular Cloning, Expression, Purification, and Characterization of Methionine Aminopeptidase
Abstract: MAP enzyme can efficiently remove N-terminal methionine from recombinant proteins. In the present study, MAP gene from genomic DNA of Escherichia coli was amplified, cloned into pET 11b vector and transformed into E.coli DH5a cloning strain to confirm the insertion of MAP gene, then plasmid was transformed into E. coli BL21 (DE3) expression strain. The protein was eluted with 25 mM NaCl and showed a purity of ~ 95% which …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 2, Issue 2, 2012 · pp. 12–24 Read article