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31 articles for “Electrophoresis”
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Existence of Integron I and II Genes from Clinical Isolate Staphylococcus aureus and Their Role in Drug Resistance
Abstract: An integron is a gene capture and dissemination system. They have been identified on both genomic and mobile genetic elements which often contain one or more genes that encode antibiotic resistance. They are commonly associated with bacterial genera in the family of Enterobacteriaceae. Currently, there have been recent reports showing the presence of integrons in Staphylococcus species. The aim of the present study was to assess the existanxe of Class …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 4, Issue 3, 2014 · pp. 18–24 Read article
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Detection of Listeria monocytogenes in Milk and Ice Cream by Polymerase Chain Reaction
Abstract: Abstract The present study was undertaken to standardize PCR assay for detection of L. monocytogenes and Listeriolysin O from livestock foods and compare its efficacy with conventional cultural methods. A set of primer derived from iap gene and other set derived from hlyA gene were used for detection of L. monocytogenes and Listeriolysin O in the PCR assay. The PCR assay was initially standardized by optimizing the concentration of the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 24–35 Read article
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Differentiation of Hybrid (COTH2) and Wild (CO3) Varieties of Tomato (Lycopersicon esculentum) Using Protein and Peroxidase Isozyme Profile
Abstract: The protein profile by SDS-PAGE and the native PAGE profile of the isoenzyme peroxidase of a hybrid tomato plant (Lycopersicon esculentum) variety (COTH2) and wild-type plants (CO3) were compared in order to adopt them as markers to differentiate them. The hybrid plant, which were resistant to leaf curl disease, was found to have higher protein content compared to its wild variety in the leaf extracts of the plants. Presence of …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 1, Issue 2, 2011 · pp. 1–5 Read article
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Semi-quantitative Event Specific Detection of Bt Maize (Event MON 810) using Real-Time PCR with SYBR Green Ι Chemistry
Abstract: The objective is this work is to do semi-quantitative analysis for Bt maize (event MON 810) using conventional Polymerase Chain Reaction (PCR) and Real Time PCR (SYBR green chemistry) from the fresh maize samples chosen arbitrarily from various locations. The protocol for extraction of good quality genomic Deoxyribonucleic acid (DNA) was standardized without the use of Liquid nitrogen. The DNA used for further studies were obtained by using standardized protocol. …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 3, 2012 · pp. 25–34 Read article
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Review on Advanced Molecular Diagnostic Tools and Their Applications
Abstract: Although the diagnosis of diseases is still primarily based on conventional methods (staining, culture, biochemical analysis), a series of advanced molecular diagnostic tools are increasingly introduced and incorporated in the workflow of clinical laboratories worldwide. These tools, such as polymerase chain reaction, northern blotting, restriction length polymorphism, flow cytometry, southern blotting, fluorescent in situ hybridization, agarose gel electrophoresis and pyrosequencing that are based on either the properties of nucleic acids …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 3, Issue 3, 2013 · pp. 21–33 Read article
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A Brief Introduction on the Electrokinetic Mixing and Separation in Microfluidic Systems
Abstract: Electrokinetics encompasses the analysis of fluid or particle motion in the electrical field; includes electroosmosis, electrophoresis, dielectrophoresis, electro-wetting, etc. Electrokinetic applications in the production of microfluidic systems have become quite attractive over the last decade. Electro-kinetic systems usually do not need any additional mechanical moving parts and may be made compact by removing the power source with a small battery. Electro-kinetic based microfluidic systems may also serve as a feasible …
Published in Journal of Aerospace Engineering & Technology · Vol. 10, Issue 3, 2020 · pp. 24–34 Read article
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Efficiency of Garamycin for the Control of Bacterial Disease: Flacherrie in the Larval Instars of Silkworm, Bombyx mori (L) (Race: Double Hybrid)
Abstract: Flacherrie, the bacterial disease is the most significant parameter associated with the loss of silk yield. The loss of appetite; discharge watery feces and vomiting are the common symptoms of infection of bacteria to the larval instars of silkworm, Bombyx mori (L). The present attempt is dealing with utilization of antibiotic compound for the control of bacterial disease: flacherrie in the larval instars of silkworm, Bombyx mori (L) (Race: Bivoltine …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 11, Issue 2, 2020 · pp. 1–16 Read article
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HPTLC: An Emerging Technique in Herbal Medicine
Abstract: Herbal medicine and their formulations are extensively used since time immemorial in developing and well developed countries. Their quality assessment is of benefit not only in research but also in practice. However, quality control of these herbo-mineral preparations is more difficult than that of conventional drug due to chemical complexity and natural variability of product and raw materials. Furthermore, the active principles are, in most cases unknown and selective analytical …
Published in Research & Reviews : Journal of Herbal Science · Vol. 4, Issue 2, 2015 · pp. 25–37 Read article
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Analytical Method Development for Quality Control and Standardization of Medicinal Plants: A Critical Review
Abstract: The demand for herbal medicine is increasing as herbs are less toxic, affordable, and culturally acceptable than the conventional drugs in developing countries. Thus, the increase in demand for standardization to ensure safety, purity and quality of medicinal plant. Standardization is a process of confirming herbal medicine identity and determination of its quality and purity. It involves botanical, physical, chemical and biological evaluations. These require application of different analytical methods …
Published in Research & Reviews : Journal of Herbal Science · Vol. 10, Issue 1, 2021 · pp. 9–20 Read article
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Amplification of 16s rRNA Gene of Brucella melitensis Causing Brucellosis Isolated from the Aborted Fetes of Goat
Abstract: ABSTRACTBrucella melitensis is zoonosis caused by facultative intracellular bacteria of genus Brucella, which are widely distributed in both human and animals, especially in developing world. The diagnosis of goat brucellosis requires isolation of the bacteria from stomach contain aborted fetes of goat. However cultural sampling sensitivity often low, depending on the disease stage, Brucella species, culture medium, quantity of circulating bacteria and blood culture technique employed. The development of PCR …
Published in Research and Reviews : Journal of Veterinary Science and Technology · Vol. 9, Issue 1, 2020 · pp. 8–12 Read article
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Transethosomes: Rising Technology for Enhancing Transdermal Delivery of Drugs
Abstract: The skin has a total surface area of around 1.8 m2 and serves as the human body's interface with the outside world. The transdermal method of medication delivery has sparked a lot of interest in pharmaceutical research since it eliminates a lot of the issues that come with the oral route. Despite the fact that the skin, especially the stratum corneum, is a barrier to most medication absorption, it does …
Published in Trends in Drug Delivery · Vol. 8, Issue 3, 2023 · pp. 57–63 Read article