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387 articles for “isolated”
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Production of Single-domain Antibodies Library by Phage Display Technology
Abstract: Single-domain antibodies (dAbs) are synthesized from variable domain gene-segments of atypical camelid or shark “heavy chain antibodies” (HCAbs) by phage display technology. We have constructed a phage display library of > 5 × 107 dAb clones derived from HCAb genes in B lymphocyte repertoire of a lipopolysaccharide (LPS)-immunized Indian desert camel and isolated dAb-phage clones by panning of the library against LPS and Staphylococcus aureus exotoxins. Nucleotide and deduced amino-acid …
Published in Research and Reviews : A Journal of Immunology · Vol. 2, Issue 3, 2012 · pp. 8–13 Read article
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New Topics in Serum Copeptin Levels in Hypertension: Is There an Interrelation with Inflammatory Biomarkers?
Abstract: This study aimed to test the hypothesis that the link between serum copeptin (Cop) levels and hypertension is related to low grade of inflammation and atherosclerosis indices. This observational cross section study was done in the Department of Biology, College of Science at the University of Al-Mustansiriya in Baghdad, Iraq. A total number of 18 healthy subjects (Group I) and 66 patients (Group II) were enrolled in this study. Patients …
Published in Research and Reviews : A Journal of Immunology · Vol. 6, Issue 2, 2016 · pp. 20–28 Read article
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Progress from the Traditional Vaccines to Novel Rational Vaccine Designs
Abstract: Vaccines are among the most valuable assets for saving human and animal lives. The vaccines discovered and designed on the Pasteurian principles laid the foundations of the traditional vaccine regime during the late-19th century. Some traditional vaccine designs proved remarkably successful, while others had safety or efficacy problems, and still others failed- particularly those against diseases caused by non-cultivable, hyper-variable or opportunist pathogens, or those requiring cellular immunity. The problems …
Published in Research and Reviews : A Journal of Immunology · Vol. 6, Issue 3, 2016 · pp. 17–31 Read article
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A Review on Current Scenario and Update on Vaccine for Covid-19
Abstract: Background: COVID-19 pandemic is caused by SARS-CoV-2 . The SARS-CoV-2 is a beta coronavirus belonging to the family of Coronaviridae. The outbreak of novel coronavirus had a major impact on health care professionals like physicians, pharmacist, microbiologist etc. Current scenario of detection of SARS-CoV-2(COVID-19) is made through a nasopharyngeal swab based on RT-PCR technique. Some novel recent studies suggest the evidence of the possible role of fluid which is present …
Published in Research and Reviews : A Journal of Immunology · Vol. 10, Issue 3, 2020 · pp. 1–7 Read article
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Development of Covid-19 Vaccine: A Review
Abstract: The emergence in December 2019 of a novel coronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has had devastating consequences globally. This is the worst catastrophe suffered by mankind in recent history. In the face of this severe disaster, people all over the world are frightened of the prospect of facing an outbreak or an annual recurrence. Control measures such as the use of masks, physical distancing, testing of …
Published in Research and Reviews : A Journal of Immunology · Vol. 11, Issue 2, 2021 · pp. 12–22 Read article
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COVID-19: An Emerging Global Threat
Abstract: The emergence of COVID-19 in Wuhan, Hubei Province, China in December 2019 has worsened global health worries. It is now declared by World Health Organization (WHO) as a Public Health Emergency of International Concern (PHEIC). Till 22 March 2021, there have been 122,524,424 confirmed cases of COVID-19 globally, with 2,703,620 deaths, and more than 216 countries are suffering from this fatal virus. The transmission of coronavirus occurs via droplets, filthy …
Published in Research and Reviews : A Journal of Immunology · Vol. 11, Issue 3, 2021 · pp. 8–19 Read article
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Pathogenesis and Treatment of Tuberculosis (T.B) Disease
Abstract: Tuberculosis (TB) is an infectious illness spread through the air that is caused by organisms from the Mycobacterium tuberculosis complex. M. tuberculosis is a lung infection in primary stage, but it can infect any area of the body. M. tuberculosis infection can progress from latent TB infection, in which the bacteria are isolated within granulomas, to infectious infection, in which the patient develops symptoms such as cough, fever, night sweats, …
Published in Research and Reviews : A Journal of Immunology · Vol. 11, Issue 3, 2021 · pp. 42–48 Read article
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RsaI polymorphisms of ER-β and Idiopathic Male Infertility
Abstract: Purpose: Study on association between estrogen receptor gene polymorphism and male infertility has been interested, but highly specific genetic markers have been reported to be variable according to regions and nations. Herein we studied to figure out whether ERβ gene1082 G>A polymorphism could influence on sperm indices or not.Method: We dealt with 100 Korean patients with idiopathic male infertility diagnosed at our university hospital as a study group and 127 …
Published in Research and Reviews: A Journal of Medicine · Vol. 12, Issue 3, 2022 · pp. 34–41 Read article
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A Study Showing the Antagonistic Effect of Lactobacillus casei Against Candida albicans
Abstract: Probiotic strain, Lactobacillus casei was isolated from the probiotic suspension named as Yakult. Strains of Candida albicans were collected from the S.N. Medical College, Agra, India. The antagonistic effect of L. casei against C. albicans was dictated by utilizing the Kirby Bauer disc diffusion technique. Maximum zone of hindrance was estimated by the serial suspension of M.F.S. ≠1.0 against C.albicans and the medium zone of hindrance was seen in the …
Published in Research and Reviews : A Journal of Medical Science and Technology · Vol. 7, Issue 2, 2018 · pp. 35–39 Read article
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Assess the Physical, Social, and Psychological Impact of Corona Viral Infectious Spread Among the General Public
Abstract: AbstractCorona, the infectious viral spread has brought many changes in our life. The physical, social and psychological impact due to long lockdown. Most of the time depression, isolation, and experiencing loneliness worsen mental health as well as physical health. The study aims to assess the physical, social and psychosocial impact of corona viral spread among the general public. The descriptive survey design and non probability purposive sampling technique was adopted. …
Published in Research and Reviews : A Journal of Medical Science and Technology · Vol. 11, Issue 3, 2022 · pp. 14–22 Read article
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Mineralization of Phenanthrene by Paenibacillus sp. PRNK-6: Effect of Synthetic Surfactants on its Mineralization
Abstract: Paenibacillus sp. PRNK-6 isolated from a soil contaminated with polycyclic aromatic hydrocarbons (PAHs) was able to utilize 69.48% of phenanthrene (320 mg l-1) in 48 h. The effect of three synthetic surfactants namely, the nonionic surfactant Tween-80, the anionic surfactant sodium dodecyl sulphate (SDS) and the cationic surfactant cetyltrymethyl ammonium bromide (CTAB) on the phenanthrene mineralization by Paenibacillus sp. PRNK-6 was studied. Results showed that the effect of each surfactant …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 7, Issue 3, 2017 · pp. 1–7 Read article
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Viral Elimination Strategies for Musa spp.
Abstract: Banana is one of the major crops in tropical parts of the world. The banana plant is susceptible to many diseases due to the less genetic diversity in its popular cultivars. Particularly viral diseases can severely destruct quantity and quality of the crop. Four major banana infecting viruses doing the damage- banana bunchy top virus, banana streak virus, banana bract mosaic virus, cucumber mosaic virus; two other viruses- banana mild …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 1, 2018 · pp. 7–14 Read article
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Fungal Synthesis of Silver Nanoparticles by an Indigenous Species of Fusarium, Its Antibacterial Activity and Efficacy as Antibiotic Enhancer
Abstract: The investigations on green combination strategies and on its potential applications for the humanity are on incline for more than 10 decades in the field of bioscience. The research on silver nanoparticles by the use of microorganisms is very much popular these days as it is very easily available source for their synthesis. In the present work, an indigenous Fusarium species isolated from soil sample has been used for the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 10–18 Read article
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Colorimetric Redox Indicator assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis in South Gujarat region
Abstract: Tuberculosis (TB) has affected humans since antiquity. The burden of TB particularly with multi-drug-resistance (MDR) is increasing worldwide and has become a major public health concern. This study aims to perform Colorimetric Redox Indicator (CRI) assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis in South Gujarat region. Total 234 samples were collected from South Gujarat region and were subjected for microscopy examination, culturing on Lowenstein-Jensen (LJ) medium and drug susceptibility …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 65–70 Read article
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A Review- Recent Outbreak of Zika Viruses in India, its Vectorology and Virology
Abstract: Zika virus is an arbovirus (arthropod-borne virus) which belongs to the family Flaviviridae that was first isolated in 1947 from blood of a febrile rhesus macaque monkey during a yellow fever study in the Zika forest of Uganda and later identified in Aedes africanus mosquito from the same forest. But in 2007, the first major outbreak of Zika virus infection occurred in Yap Island (Federated States of Micronesia), where, it …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 2, 2019 · pp. 25–33 Read article
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Prevalence of Oral Candidiasis by Candida albicans in Patients with Type 2 Diabetes Mellitus Visiting Mahakali Zonal Hospital
Abstract: Patient with type 2 diabetes mellitus are at consistent risk of oral candidiasis, dental caries and dysfunction. Candiadisis is caused by different species such as Candida albicans, C. tropicalis, C. glabrata, C. dubliniensis. Nevertheless, C. albicans is the most frequent causative agent for candidiasis in human. Present work was undertaken to investigate Candida albicans infection among type 2 diabetic mellitus patients in Mahendranagar, Kanchanpur. Methods: A total of 103 oral …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 10, Issue 1, 2020 Read article
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Prevalence of Bacterial Colonisation in Patients Undergoing Hemodialysis: Experience from a Tertiary Care Centre
Abstract: Routine surveillance of patients on hemodialysis is necessary to prevent and control skin colonization and staphylococcal infections. To assess the prevalence of bacterial colonization on various skin sites in patients undergoing hemodialysis. A cross-sectional study was conducted on adult patients at a tertiary care center of North India. Study was conducted from July 2016 to September 2016 on adult patients with newly diagnosed end stage renal disease (ESRD) on maintenance …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 10, Issue 3, 2020 · pp. 1–9 Read article
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To Study the Effects of CIMT (Constraint-Induced Movement Therapy) Vs. GRASP (Graded Repetitive Arm Supplementary Program) Training in Improvement of Arm and Hand Function in Chronic Stroke Patients: A Comparative Study
Abstract: Stroke is the leading cause of adult disability and inpatient rehabilitation admissions. An estimated 70% of the patients who survive a stroke are unable to use their limbs (UE/LL) independently during the first 3 to 4 weeks post stroke. Patients, who have suffered a stroke, experience loss, uncertainty, and social isolation, as stroke represents a sudden, overwhelming, and fundamental change for the survivor. Personal control over progress, optimism, and fears …
Published in Research and Reviews: A Journal of Neuroscience · Vol. 8, Issue 3, 2018 · pp. 24–29 Read article
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Free Fatty Acids Modulate the Specific Activity and Expression of Alpha and Beta Subunits of Sodium Potassium ATPase of Cultured Rat Cortical Astrocytes.
Abstract: The fatty acid composition of the membrane plays an important role in the functioning of the membrane-bound enzyme Na+K+ATPase. The aim of this study was to determine the effects of the free fatty acids at various levels of unsaturation, namely, stearic acid, linoleic acid, arachidonic acid and docosahexaenoic acid, on the alpha and beta subunits of sodium potassium ATPase present on the primary cultures of rat astrocyte membrane. Membrane was …
Published in Research and Reviews: A Journal of Neuroscience · Vol. 2, Issue 1, 2012 · pp. 20–28 Read article
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Patient Teaching for Cardiac Rehabilitation in Cardio Diabetes
Abstract: Cardiovascular diseases (CVDs) are the leading cause of mortality worldwide, and are a significant contributor to morbidity and health-related costs. Coronary artery disease (CAD)—the most common type of CVDs—is considered as a chronic condition and, therefore, requires a careful medical management with multiple recommendations for patients to achieve optimal secondary prevention. As a consequence, patient education is a necessary step to promote patient understanding of the recommended therapies and behavior …
Published in Research and Reviews: A Journal of Neuroscience · Vol. 7, Issue 2, 2017 · pp. 29–35 Read article