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247 articles for “assay”
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Biofilm Forming and Antimicrobial Susceptibility of Clinical Isolates of Staphylococcus Species
Abstract: Staphylococcus species are the most frequent causes of infection and infections on indwelling medical devices, which characteristically involve biofilms. This study observes the biofilm forming abilities and to analyze antimicrobial susceptibility pattern of clinical isolates of Staphylococcus species were found to be biofilm producers by microtitter plate assay method. And to evaluate the antibiofilm property of Moringa oleifera an Indian medicinal plant, known to possess antimicrobial activity. The results demonstrate …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 2, 2015 · pp. 1–4 Read article
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Helicobacter pylori Sialic Acid-Specific Surface Lectin Associated with Pathogenecity Island cag PAI
Abstract: Normal 0 false false false MicrosoftInternetExplorer4 Helicobacter pylori bacterium causing gastritis, duodenitis, non-ulcer dyspepsia, adenocarcinoma, MALT lymphoma and now-a-days causing hepatocellular carcinoma and cholengiocarcinoma has been studied for its surface antigens. There are many virulence factors which have been studied since many years. Cag-PAI is the site for virulence and causes mixed infections. Sialic acid is a type of glycan which is a surface lectin associated with adherence to cell …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 3, 2015 · pp. 10–17 Read article
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Antimicrobial Susceptibility Profile of Airborne Bacteria and Fungi at Owena Market, Osun State, Nigeria
Abstract: Environmental conditions and human activities influence the ubiquity and diversity of microorganisms in the atmosphere thereby acquiring the ability to resist antibiotics which poses serious public threat. Airborne bacteria and fungi isolated using settling plate method from three different points (where kolanuts, meat and onions are sold) at Owena Market, Osun state, Nigeria were assayed for antimicrobial susceptibility using disc diffusion. The microorganisms were identified as Staphylococcus aureus, Bacillus subtilis, …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 7, Issue 2, 2017 · pp. 12–16 Read article
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Green Synthesis and Characterization of Silver Nanoparticle using Leaves of Lawsonia inermis: Antibacterial, Antifungal and Anti-Oxidant Activity
Abstract: Nanotechnology is one of the fastest developing sciences over the past few years. This is an emerging field of modern research dealing with synthesis and designing of particle structure ranging from approximately 1–100 nm. Silver nanoparticles (AgNPs) are known for their tremendous applications in the field of therapeutics and diagnosis. In the present study, we have confirmed that the antibacterial, antifungal and anti-oxidant activity of biosynthesized AgNPs which were synthesized …
Published in Journal of Nanoscience, NanoEngineering & Applications · Vol. 6, Issue 1, 2016 · pp. 11–23 Read article
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Chemotaxis: role in egg-sperm interaction and its evaluation
Abstract: Communication between spermatozoa and egg before contact by chemotaxis is prevalent throughout the animal kingdom. In most animal species, the prospects of the spermatozoa coming in contact with eggs are very slim in the absence of some guidance mechanism usually chemical in nature that has been acquired gradually. Such chemical guidance, sperm chemotaxis, is now recognized in many marine invertebrates, fish, amphibians, and a few mammals including humans. This suggests …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 1, Issue 1, 2011 · pp. 1–21 Read article
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L-glutaminase of Vibrio sp. from White-Spot Virus disease out-broken shrimp pond
Abstract: Bacteria from White Spot Viral (WSV) disease out-broken shrimp (Penaeus monodon) pond sediments located in the bank of Vellar estuary, South east coast of India were enumerated, enriched, screened by plate assay and confirmed as Vibrio sp. through biochemical tests. Extracellular L-glutaminase was eluted from Vibrio sp. by centrifugation and purified by downstream process. The final yield was 1.7% with 15.4 IU mg-1 of specific activities, which was 17.2 fold …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 1, Issue 1, 2011 · pp. 22–27 Read article
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¬¬¬Lycopene Protects Testes and Epididymal Sperm in BPA Exposed Adult Sprague-Dawley Rats
Abstract: Bisphenol A (BPA) is ubiquitous and persistent environmental contaminant. Previous studies proved that BPA disrupt hypothalamic–pituitary–gonadal axis and oxidative balance. Numerous studies have clearly been reported that oxidative stress leads to male infertility by affecting testes and spermatozoa. On the other hand, lycopene has beneficial role on testes and spermatozoa by reducing the oxidative stress. Therefore, this study was conducted to delineate the protective role of lycopene by acting against …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 1, Issue 1, 2011 · pp. 101–107 Read article
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Cytotoxicity of Titanium Dioxide Nanopaprticleson A Bacterial Population Isolated from Lakewater Sediments
Abstract: Studies suggest that approximately 4.6 million ton of pigmentary titanium dioxide (TiO2) is consumed annually worldwide. Owing to the increased industrial and consumer applications, there is growing concern regarding the possible risk associated with their environmental exposure. TiO2 dust, when inhaled, has been classified by the International Agency for Research on Cancer (IARC) as an IARC Group 2B carcinogen, meaning it is possibly carcinogenic to humans. TiO2 nanoparticles (NPs) can …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 31–35 Read article
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Comparative Assessment of Antiproliferative Potential of Triphala and Ashokarishta: Dependence on Dose, Exposure Time and Age of the Preparation
Abstract: Triphala and Ashokarishta are known to be widely used herbal formulations in Ahmedabad, of which a few are local brands. Triphala is used by people of all ages for general well being while Ashokarishta is prescribed to women suffering from problems related to menstruation. Five brands of Tripahala and three brands of Ashokarishta were added to Schizosaccharomyces pombe NCYC 1683 cultures (cell cycle mutants) at concentrations of 0.5, 1.0, 1.5 …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 2, 2014 · pp. 8–16 Read article
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Clinical Implications of Serum NT-pro Brain Natriuretic Peptides in Type1 Diabetes with Positive Antiviral Antibodies
Abstract: Subclinical cardiovascular disease is common in diabetes mellitus and is associated with an increased risk of cardiovascular events including heart failure. This study was aimed to assess the association of NT-proBNP as a marker of heart failure and antiviral antibodies in patients with type 1 diabetes mellitus (T1D). One hundred and forty-seven T1D patients (65 male and 82 female) and 24 (11 male and 13 female) non-diabetic control subjects were …
Published in Research and Reviews: A Journal of Medicine · Vol. 3, Issue 1, 2013 · pp. 12–16 Read article
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In Vitro Screening of Antibacterial Potentiality of Some Important Flowers’ Extracts Against Some Pathogenic Bacteria
Abstract: Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 The present investigation was carried out to screen and evaluate the antibacterial activities of different flowers, namely, Calendula officinalis, Rosa damacena, Hibiscus rosa-sinensis, Datura stramonium, Bougainvillea stabilis, and Vinca rosea. These flowers were screened for potential antibacterial activity against some important bacterial Gram negative and Gram positive bacteria strains, namely Escherichia coli, Salmonella typhi, Staphylococcus aureus, and E. faecalis. Dried leaf …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 1, Issue 3, 2011 · pp. 9–19 Read article
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Production of Phosphatase by Phosphate Solubilizing Improved Fungal Strains
Abstract: Phosphate solubilizing micro-organisms were isolated from rhizosphere soils of rice fields around Mannachanalur area, Tiruchirappali district of Tamilnadu. The isolated fungal cultures were identified as Aspergillus niger, Aspergillus fumigatus and Penicillium sp. The efficacy of Phosphatase activity of fungal strains was carried out. The fungal strains were subjected to random mutational studies such as UV and chemical mutation. The Phosphatase assay was carried out after random mutagenesis using wild and …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 2, Issue 1, 2012 · pp. 39–52 Read article
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Changes of Super Oxide Dismutase Activity in Hemolymph, Hemocytes and Fat Body of Silkworm (Bombyx mori L.) Challenged by Micrococcus luteus and Escherichia coli
Abstract: The hemolymph, hemocytes and fat body of the silkworm, Bombyx mori L. were determined for superoxide dismutase (SOD) activity following bacteria Micrococcus luteus and Escherichia coli challenged using a photochemical assay system consisting of methionine, riboflavin, and p-nitro blue tetrazolium. The in vitro reduction of nitro blue tetrazolium by superoxide to formazan was measured spectrophotometrically in Bombyx Mori. Hemolymph, hemocytes and fat body were in response to various immune elicitors. …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 2, Issue 2, 2012 · pp. 1–9 Read article
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Preparation of Lipase Loaded Chitosan Nanoparticles and its Estimation Using Different Additives
Abstract: Chitosan nanoparticles were prepared by ionic gelation technique and simultaneously entrapment of lipase was carried out in it. These were then subjected to additives such as surfactants, pluronics and ionic liquids. Activity of lipase was assayed in free as well as immobilized conditions. It was then compared to immobilized enzyme bound to these polymers. The results have been graphically displayed. Among surfactants, tween 80 and triton X-100 were used with …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 6, Issue 2, 2016 · pp. 19–27 Read article
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Thyroid Disorders in Regard to Aetiology, Clinical Patterns, Management
Abstract: Thyroid disorders are one of the important and common endocrine problems encountered in clinical and occupational practice in all age groups more frequently in women and in adult population. Amongst all the functional thyroid disorders, hypothyroidism is far more common than hyperthyroidism. Recognition of this condition at an early stage can delay the progression to overt disease. Appropriate use of biochemical test, i.e., thyroid function test can delineate practically all …
Published in Journal of Energy, Environment & Carbon Credits · Vol. 6, Issue 3, 2016 · pp. 34–42 Read article
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Green Synthesis and Characterization of Silver Nano-conjugates Using Heliotropium indicum and Glycosmis pentaphylla Leaf Aqueous Extracts
Abstract: Heliotropium indicum Linn. (Family Boraginaceae), an annual herbaceous medicinal plant, is usually known as Indian heliotrope. Glycosmis pentaphylla (Retz.) DC (Family Rutaceae) is a perennial shrub, commonly known as orange-berry. These traditionally used medicinal plants originate from tropical and temperate parts of the world along with India, Southeast Asia, Northern Australia, and some other African countries. Extensive literature studies suggested that the different parts of the plant are reported to …
Published in Journal of Nanoscience, NanoEngineering & Applications · Vol. 9, Issue 2, 2019 · pp. 22–30 Read article
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Synthesis of Silver Nanoparticles from Pseuderanthemum malabaricum and Evaluation of Anticancer Activity
Abstract: The present study was focused on eco-friendly and green approach synthesis of silver nanoparticles using plant extracts of P. malabaricum. The morphological and physical properties of synthesized silver nanoparticles were analyzed using various spectroscopic and microscopic studies such as UV visible spectroscopy, FT-IR, XRD, SEM with EDAX, DLS, Zeta potential and Particle size analyzer. After structural characterization, the synthesized silver nanoparticles were subjected to anti-cancer activity studies against cervical cancer …
Published in Nano Trends – A Journal of Nano Technology & Its Applications · Vol. 21, Issue 2, 2019 · pp. 31–40 Read article
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Aerogel Cellulose Composite–Considered at Low Cost
Abstract: This review descriptive issued as from cellulose aided fabrication to form eco-friendly aerogel. This is lightweight material to contain more than 99% air, which has been processed by multi shape in structure depending on application. Susceptibility to chain linkage to adjoin by glucoside bond, cellulose has polymerized naturally or artificially. Assay of script as from beneficiated bio-compose has utilized for amidation, etherification, silylation, urethanization, etc. Mechanical property of nanocellulose has …
Published in Nano Trends – A Journal of Nano Technology & Its Applications · Vol. 23, Issue 1, 2021 · pp. 5–15 Read article
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Anti-cancerous Activity of the Rhizome Extract of Alpinia galanga L. Willd. on the Human Liver Carcinoma Cells
Abstract: The rhizome of Alpinia galanga. L. (Willd). was collected from three places i.e, Udupi Shankara narayana temple surrounding area, Shringeri and Madikeri accessions. About 100 gms of the rhizome was taken, dried and powdered to obtain 1 gm of the extract through methanol as the solvent. 1 gm of the solid extract was subjected for filteration through millipore syringe filter., to this about 6ml of cell based assay buffer and …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 8, Issue 2, 2018 · pp. 1–6 Read article
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In vitro Antibacterial Activity of Averrhoa carambola Leaf Extracts
Abstract: The aim of the present study was to determine the phytochemical constituents and antimicrobial potential of Averrhoa carambola leaf extracts against three gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Streptococcus mutans) and three gram-negative bacteria (Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa) which are some of the infectious bacteria. The A.carambola leaves were extracted in three different solvents of increasing polarities (hexane, ethyl acetate, and acetone). The phytochemical analysis showed …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 8, Issue 2, 2018 · pp. 27–32 Read article