Green Synthesis and Characterization of Silver Nanoparticle using Leaves of Lawsonia inermis: Antibacterial, Antifungal and Anti-Oxidant Activity

N Durga; K. Venkateswara Rao; CH. Shilpa Chakra; T. Dayakar; N. Jaya Rambabu

Abstract

Nanotechnology is one of the fastest developing sciences over the past few years. This is an emerging field of modern research dealing with synthesis and designing of particle structure ranging from approximately 1–100 nm. Silver nanoparticles (AgNPs) are known for their tremendous applications in the field of therapeutics and diagnosis. In the present study, we have confirmed that the antibacterial, antifungal and anti-oxidant activity of biosynthesized AgNPs which were synthesized by using the aqueous extract of Lawsonia inermis (Henna or Mehandi). The size and morphology of biosynthesized silver nanoparticles was monitored by x-ray diffraction, Fourier transform infrared spectroscopy, UV-Vis spectrophotometer, scanning electron microscopy and transmission electron microscopy. The leaves were found to be a reducing agent as well as a stabilizing agent. Antibacterial and antifungal activity was estimated by disc diffusion method, antioxidant activity of AgNPs was determined by DPPH and reducing power assay. This nanoparticle has shown balancing and inhibiting the reactive oxygen species (ROS) generation scavenging free radicals. From the results, it was found that the biologically synthesized AgNPs have higher antifungal than antibacterial and also to have higher antioxidant activity. Keywords: Silver nanoparticles, Lawsonia inermis, antibacterial, antifungal activity, anti-oxidant activity