Antibacterial Action of Bacillus coagulans Against Diverse Pathogens and its Relevance as Probiotics

Monika Gujjar; Sakshi Tewari

doi:10.37591/rrjomv.v13i2.3262

Abstract

Probiotics are living organisms that give benefits to the host organism by improving the intestinal microbial balance. Probiotics have various beneficial effects as it maintains a healthy gut, healthy vaginal flora and boost the immune system. There are different strains of lactic acid bacteria that are used as probiotics but Bacillus coagulans is a good bacterium that is used in the form of probiotic supplements. In the present study, B. coagulans was isolated from the probiotic medicine named Sporlac-DS using De Man, Rogosa and Sharpe agar medium at 37°C for 24 h. Isolated strain was monitored for its morphological, biochemical, and physiological examinations under in vitro conditions. It was monitored that the strain was gram-positive, rod-shaped bacteria, and displayed high milk curdling activity. Biochemical analysis proved that the strain was catalase-positive and fermented glucose and lactose. The antibacterial activity of the isolated strain was further confirmed when its inhibitory potential was observed against test organisms like Pseudomonas, Staphylococcus, and Escherichia coli by well diffusion assay. Clear zones of 8 mm, 10 mm, and 7 mm diameter, respectively, were observed when Pseudomonas, Staphylococcus, and E. coil were spread on the plate, respectively. The reason for this inhibition may be the diverse antagonistic mechanisms working in synchrony viz. lactic acid + bacteriocins. Hence, the study recommends the usage of Sporlac tablets due to their antimicrobial action against diverse pathogens and boost the immune system. Currently, Sporlac is taken during constipation, irritable bowel syndrome, diarrhea, gas, indigestion, and many other conditions, but there is also evidence that intake of Sporlac helps in treating bacterial vaginosis in females.

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