Transient Heat Shock Induces Oxidative Stress in Mice Testes. Amelioration by Selenium and Vitamin E

Sarvnarinder Kaur; M.P. Bansal

Abstract

The present study evaluated the role of Selenium (0.5 ppm selenium/kg diet) and Vitamin E (200 mg alpha-tocopherol/kg diet) (as antioxidants) in modulating the oxidative stress induced during transient heat shock (42°C-30 mins) in mice testes. Study included six different groups of male Balb/c mice; Control, Heat shock, Selenium, Selenium+heat shock, Vitamin E and Vitamin E+heat shock. Sperm parameters viz. sperm concentration and sperm motility, LPO and ROS levels, GSH, GSSG and redox ratio were evaluated. Further, histological analysis was done using light microscopy and testicular cell kinetics study was carried out. A significant decrease in sperm parameters was seen in heat shock group in comparison to control group. Elevation in LPO and ROS levels and reduction in redox ratio along with morphological alterations in testes at light microscopy level was observed in Heat Shock group. These observations clearly indicate the development of oxidative stress condition after scrotal hyperthermia. Further, it was observed that selenium and Vitamin E supplementation to heat shock treated animals played a protective role against oxidative stress generated by heat shock. Present study therefore could be of therapeutic importance in cases with scrotal hyperthermia; however, further analysis of the molecular targets involved would give a better understanding of the mechanism involved. Keywords: Spermatogenesis, antioxidants, selenium, vitamin E, oxidative stressCite this ArticleSarvnarinder Kaur, M.P. Bansal. Transient Heat Shock Induces Oxidative Stress in Mice Testes. Amelioration by Selenium and Vitamin E. Research & Reviews: A Journal of Life Sciences. 2019; 9(2): 17–31p.