Antifungal Susceptibility Pattern of Candida Species Isolates from Dehradun City Hospitals

Manshi Gusain

Abstract

The present investigation on the topic entitled “Antifungal susceptibility analysis of Candida species isolates from Dehradun City hospital.” was accomplished in the Doon University Microbiology laboratory (P.G.) and Hospital, Dehradun, Uttarakhand, during the period of February 2021 to July 2021. A total of 42 clinical samples were collected from patients, suspected of candidiasis from Dehradun city hospitals. Clinical samples were collected from oral cavity, blood, vaginal fluid and urine of the patients. Among them 24 yeasts were obtained. These were identified and characterized by germ tube formation, Microscopic, morphological and biochemical test. Out of 24 Candida isolates, Candida albicans was the commonest 16 (66.66%). Other non albicans species were 8 (33.33%). Out of 8 non albicans species 5 (62.5%) were C. tropicalis and 3 (37.5%) were C. glabrata. Prevalence of Candida in urine samples were found comparatively higher. We have studied 16 urine samples out of them 13 (81.2%) yeasts isolates representing 3 Candida species were found. The isolates 8 contained C. albicans as the most prevalent species (50 percent), after by C.tropicalis 3 (18.7%) and C. glabrata 2 (12.5%). We have conducted antifungal sensitivity test for 24 isolates of yeasts. According to CLSI recommendations, antibiotic sensitivity evaluation was carried out using Disc Diffusion (DD) method.Using fluconazole discs 8 (33.3%) of the 24 yeasts isolates were classified as S, 10 (41.6%) were S-DD and 6 (25%) were resistant. Using Itraconazole discs 6 (25%) of the 24 yeast isolates were classified as S, 10 (41.6%) were S-DD and 8 (33.3%) were resistant. Using Clotrimazole discs (10µg) 8 (33.3%) of the 24 yeast isolates were classified as S, 15 (62.5%) were S-DD and 1 (4.16%) were resistant. Using ketoconazole discs 23 (95.8%) of the 24 yeast isolates were classified as S, 1 (4.16%) were S-DD and No one was resistant. Using amphotericin B discs 14 (58.3%) of the 24 yeast isolates were classified as S, 5 (20.8%) were S-DD and 5 (20.8%) were found resistant. Candida species isolates showing 33.3%, 25.0% resistance against itraconazole and fluconazole respectively. Systemic infection due to yeast and resistance to antifungal is on rise in Indian population.

Abstract

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