The present study aimed to identify some of the phytoconstituents of a fraction (fraction DDK-4) obtained from the column chromatography of the ethylacetate extract of Securidaca longipedunculata Fresen (Polygalaceae). Liquid Chromatography-Mass Spectrometry (LCMS/MS) analysis of fraction DDK-4 was conducted on a Waters Synapt G2 quadrupole time-of-flight mass spectrometer (Milford, MA, USA) using positive mode of ionization. The results showed that 1,7-dihydroxy-2,8-dimethoxy xanthone, 3-(1,3-benzodi-oxol-5-yl-4-morpholinyl)-3-(2-hydroxy-4,6-dimethoxyphenyl)-1-propanone, 5,7-dihydroxy-6-methyl-3-(2’,4’-dihydroxybenzyl)-chroman-4-one, trans-O-methyl-grandmarin, dioctylamine, rhodioloside, punicic acid, echimidine, N-[(2R,3S)-2-[[(3,5-dimethyl-4-isoxazolyl)sulfonyl-methylamino]methyl]-5-[(2R)-1-hydroxypropan-2-yl]-3-methyl-6-oxo-3,4-dihydro -2H-1,5-benzoxazocin-10-yl]-1,3-benzothiazole-2-carboxamide, scilliroside and geranyl hydroquinone were identified as the phytoconstituents present in fraction DDK-4. The presence of these compounds in the stem bark of S. longipedunculata has been reported for the first time. Also, 1,7-dihydroxy-2,8-dimethoxy xanthone could be very useful in the chemotaxonomy of the genus Securidaca and family Polygalaceae. Studies are currently on-going to isolate these compounds in their pure forms in order to elucidate and characterize their structures using different spectroscopic techniques.
