Studies of CRISPR/CAS 9 Gene Interactions in Streptococcus pyogenes

D.S.V.G.K. Kaladhar

doi:10.37591/rrjocb.v7i3.627

Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/cas9/single guide RNA (sgRNA) system is a DNA editing system in mostly studied in prokaryotes. Studies of CRISPR/cas9 gene interactions in Streptococcus pyogenes has been conducted using in silico approaches in the present research. Proteins like DNA polymerase I (polA), NAD-dependent DNA ligase LigA (ligA), Translaldolase (tal), Thymidine kinase (tdk), Streptococcal exotoxin I (speI), Phage associated protein (SPy_0679) and Hypothetical proteins like SPy_1047, SPy_1048, csn2 and SPy_2034 has shown interaction with cas9. The streptococcus pyogenes has shown relevance with Microbacterium arborescens and Klebsiella pneumonia based on cas9 protein sequence homology. Further in vitro and in vivo research has to be conducted for better understanding to propose the mechanism. Keywords: CRISPR, Cas9, Streptococcus pyogenesCite this ArticleD.S.V.G.K. Kaladha. Studies of CRISPR/CAS 9 Gene Interactions in Streptococcus pyogenes. Research & Reviews: Journal of Computational Biology. 2018; 7(3): 32–38p.