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Research & Reviews : Journal of Botany Original Research

Therapeutic Prospects and Comparative Bioactive Evaluation of Fenugreek Seed Extracts

  1. Ankit Kumar Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida-201309

Abstract

A medicinal plant with substantial therapeutic potential, fenugreek (Trigonella foenum-graecum L.) is rich in bioactive phytoconstituents. The phytochemical makeup and antioxidant properties of aqueous and methanolic fenugreek seed extracts were compared in this study. Phenolics, flavonoids, terpenoids, alkaloids, tannins, and saponins were found in both extracts, with the methanolic fraction showing greater intensity, according to preliminary phytochemical screening. The methanolic extract has higher levels of phenolic, flavonoid, and terpenoid content, according to quantitative analyses. Antioxidant analysis showed that the methanolic extract had better reducing potential and lipid peroxidation inhibition than the aqueous extract. Additionally, the methanolic extract showed increased capacity to scavenge free radicals from DPPH, ABTS, hydrogen peroxide, nitric oxide, superoxide, and hydroxyl radicals. The richer phytochemical composition of the methanolic extract may be responsible for its increased bioactivity. Overall, the results demonstrate the therapeutic value of fenugreek seed extracts, especially the methanolic extract, for possible phytopharmaceutical and nutraceutical uses in conditions linked to oxidative stress

Keywords

References (16)

  1. khan F, Negi K, Kumar T. Effect of sprouted fenugreek seeds on various diseases: a review. Journal of Diabetes, Metabolic Disorders & Control. 2018;5(4):119-125. doi:10.15406/jdmdc.2018.05.00149
  2. Dahab AA, Bayomy HM, El-Salam HSA, Almasoudi SE, Ozaybi NA, Mahmoud GA, et al. Seed Disinfection Treatments Minimized Microbial Load and Enhanced Nutritional Properties of Fenugreek Sprouts Which Alleviated Diabetes-Negative Disorders in Diabetic Rats. Nutrients. 2024;16(16):2635. doi:10.3390/nu16162635
  3. Chauhan G, Sharma M, Varma A, Kharkwal H. Phytochemical analysis and anti-inflammatory potential of fenugreek. Med Plants Int J Phytomed Relat Ind. 2010;2(1):39–44. doi:10.5958/J.0975-4261.2.1.005.
  4. Singh P, Bajpai V, Gond V, Kumar A, Tadigoppula N, Kumar B. Determination of Bioactive Compounds of Fenugreek (Trigonella foenum-graecum) Seeds Using LC-MS Techniques. Methods in Molecular Biology. 2020:377-393. doi:10.1007/978-1-0716-0235-5_21
  5. Inanmdar W, Sultana A, Mubeen U, Rahman K. Clinical efficacy of Trigonella foenum graecum (Fenugreek) and dry cupping therapy on intensity of pain in patients with primary dysmenorrhea. Chinese Journal of Integrative Medicine. 2016. doi:10.1007/s11655-016-2259-x
  6. Benayad Z, Gómez-Cordovés C, Es-Safi N. Characterization of Flavonoid Glycosides from Fenugreek (Trigonella foenum-graecum) Crude Seeds by HPLC–DAD–ESI/MS Analysis. International Journal of Molecular Sciences. 2014;15(11):20668-20685. doi:10.3390/ijms151120668
  7. Kumar P, Bhandari U, Jamadagni S. Fenugreek Seed Extract Inhibit Fat Accumulation and Ameliorates Dyslipidemia in High Fat Diet-Induced Obese Rats. BioMed Research International. 2014;2014:1-11. doi:10.1155/2014/606021
  8. Katalinic V, Milos M, Kulisic T, Jukic M. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry. 2006;94(4):550-557. doi:10.1016/j.foodchem.2004.12.004
  9. Schieber A, Keller P, Carle R. Determination of phenolic acids and flavonoids of apple and pear by high-performance liquid chromatography. Journal of Chromatography A. 2001;910(2):265-273. doi:10.1016/s0021-9673(00)01217-6
  10. Wong CC, Li HB, Cheng KW, Chen F. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chemistry. 2006;97(4):705-711. doi:10.1016/j.foodchem.2005.05.049
  11. Shakuntala S, Pura Naik J, Jeyarani T, Madhava Naidu M, Srinivas P. Characterisation of germinated fenugreek (Trigonella foenum-graecum L.) seed fractions. International Journal of Food Science & Technology. 2011;46(11):2337-2343. doi:10.1111/j.1365-2621.2011.02754.x
  12. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999;26(9-10):1231-1237. doi:10.1016/s0891-5849(98)00315-3
  13. Benzie IFF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry. 1996;239(1):70-76. doi:10.1006/abio.1996.0292
  14. Hancock JT, Neill SJ. Nitric Oxide: Its Generation and Interactions with Other Reactive Signaling Compounds. Plants. 2019;8(2):41. doi:10.3390/plants8020041
  15. HOODA S, JOOD S. EFFECT OF SOAKING AND GERMINATION ON NUTRIENT AND ANTINUTRIENT CONTENTS OF FENUGREEK (TRIGONELLA FOENUM GRAECUM L.). Journal of Food Biochemistry. 2003;27(2):165-176. doi:10.1111/j.1745-4514.2003.tb00274.x
  16. Navarro del Hierro J, Reglero G, Martin D. Chemical Characterization and Bioaccessibility of Bioactive Compounds from Saponin-Rich Extracts and Their Acid-Hydrolysates Obtained from Fenugreek and Quinoa. Foods. 2020;9(9):1159. doi:10.3390/foods9091159
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