Biofilm is the deposition of microorganisms and their products on a solid surface submerged in water, which is prevalent problem, responsible for the degradation of the surface over the time. Elimination of microorganisms from submerged solid surface is enormously important process to reduce degradation of that surface. Recently, Tin Oxide (SnO2) nanoparticles have attracted the attention of researchers for its antibacterial property due to hydrophobic and non-toxic nature. Thin films of tin oxide have potential to serve as nano-structured material against biofilm formation that requires less material and leads to cost effectiveness. This paper presents deposition of thin film of tin oxide via (TVC) Thermal Vacuum Coating method on a glass substrate. The vacuum pressure during deposition was 5x10–5 mbar and the distance between substrate to boat was 20 cm. The rate of deposition was 10 ±2 Å/ Sec. at current in the range of 150 Ampere. The thickness of the deposited film was 300 nm. The structural and material characterisation of the thin film was carried out using XRD and FTIR. Optical properties were studied using UV-VIS Spectrophotometer. The fluorescence microscopy was used to observe the antibacterial biofilm activity of tin oxide thin film against bacterial strains-E. coli and S. aureus and it was found that thin film of tin oxide has significant antibacterial biofilm activity against these bacteria. Keywords: biofilm, thin film, antimicrobial activity, tin oxideCite this Article Atul Kumar, Ravish Garg, Rakesh Dhar, et al. Antibiofilm Activity of Thermal Evaporated Tin Oxide Thin Film. Journal of Nanotrends. 2015; 17(3): 1–5p.