Research and Reviews : A Journal of Biotechnology
Volume 4, Issue 1 (2014)
Published
Table of contents
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Production and Characterization of Thermostable Amylase Produced from Spore Former Thermophilic Bacilli Isolated and Screened from Teff (Eragrostis teff) Starch Rich Soil
Abstract: There is a need to isolate and screen amylase-producing bacilli bacteria from soil rich in content of teff starch at high temperature environment. Therefore, the aim of this study was to isolate and screen amylase-producing bacilli isolates and characterization of their optimal amylase activities. Amylase-producing bacilli were isolated and their enzymes characterized. Effect of temperature, pH, substrate and salt concentration were determined. All isolates have clear zone over 15 mm …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 1–11 Read article
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Study of Seed Germination and Protein Content of Four Varieties of Glycine max L. – A Miracle Crop
Abstract: Glycine max L. (Soybean) is an important source of high-quality proteins, fats, vitamins, minerals and other nutrients for both human beings and animals. The study was investigated on the seed germination, purity percentage and protein content of four varieties of Glycine max L. namely, JS335, JS9560, JS9305 and PK1042, collected from the Indian Agriculture Research Institute, Pusa, New Delhi (India). The experimental data showed that variety JS335 exhibited 85% germination, …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 12–14 Read article
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Insecticidal Activity of Rotenone in Brebra (Millettia Ferruginea) Oil Refinery Residue against Different Stored Crop Insect Pests and its Prospect to Apply as Pest Control Substance
Abstract: Use of botanical insecticides against stored crop insect pests is environmental safer and eco-friend alternative method for crop protection. The objective was to investigate insecticidal potential of oil refinery residue with rotenone against selected insect pest of stored crops. The amount of rotenone from samples was determined by HPLC. The toxicity of the rotenone from residual extract and non-defatted sample were tested against. Acanthoscelides obtectus, Sitophilus zeamais and Tribolium castaneum …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 15–23 Read article
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Designer Zinc Finger Nuclease: A Versatile Tool for Genome Editing in Animals
Abstract: In this review, is provided an update on the most popular approaches, including zinc finger nuclease (ZFN)-based gene editing by homologous recombination (HR), gene knockout, non-homologous end joining (NHEJ), and gene therapy in mouse. Zinc finger nucleases (ZFNs) are synthetic restriction enzymes evolved by fusing zinc finger that facilitate genome editing by creating a double-stranded break in DNA at a user-specified location. ZFNs are new tools that promise to radically …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 24–30 Read article
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Cytotoxicity of Titanium Dioxide Nanopaprticleson A Bacterial Population Isolated from Lakewater Sediments
Abstract: Studies suggest that approximately 4.6 million ton of pigmentary titanium dioxide (TiO2) is consumed annually worldwide. Owing to the increased industrial and consumer applications, there is growing concern regarding the possible risk associated with their environmental exposure. TiO2 dust, when inhaled, has been classified by the International Agency for Research on Cancer (IARC) as an IARC Group 2B carcinogen, meaning it is possibly carcinogenic to humans. TiO2 nanoparticles (NPs) can …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 31–35 Read article
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Forward Osmosis Membrane Concentration Process of Anthocyanins from Pomegranate Fruit
Abstract: The production of concentrated pomegranate juice was investigated by using forward osmosis membrane concentration process. Suspended solids were completely removed in the clarification step, while soluble solids and organic acids were concentrated using forward osmosis membrane process. The processes was performed at ambient temperature (25 ± 2 °C) and non pressure driven process to produce a concentrated extract to a total soluble solids (TSS) content of 60ï‚° Brix from an …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 36–41 Read article
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Production and Characterization of Biosurfactant from Indigenous Bacterium Bacillus amyloliquefaciens USTBb: Feasibility in Microbial Enhanced Oil Recovery
Abstract: In the present study, the production and characterization of biosurfactant by an indigenous Bacillus methylotrophicus USTBb (GenBank accession no.KF496216) was investigated. This bacterial strain was isolated from crude oil contaminated aqueous medium was identified to be a persuasive producer of biosurfactant using crude oil as the sole carbon and energy source. Growth parameters were optimized, with the aim of increasing biosurfactant productivity. Maximum biosurfactant production (5.5 g L-1) by strain …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 42–55 Read article