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Journal of Polymer & Composites Original Research Special issue Open Access

Examining Connective Tissue Changes Across Various Grades of Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma: A Histochemical Polymeric Investigation

  1. Fazilram P Department of Oral Pathology, School of Dental Sciences, Krishna Vishwa Vidyapeeth Deemed to be University, Karad,
  2. Dr Uzma Belgaumi , Department of Oral Pathology, School of Dental Sciences, Krishna Vishwa Vidyapeeth Deemed to be University, Karad,
  3. Dr. Nupura Vibhute , Department of Oral Pathology, School of Dental Sciences, Krishna Vishwa Vidyapeeth Deemed to be University, Karad,
  4. Dr. Vidya Kadashetti , Department of Oral Pathology, School of Dental Sciences, Krishna Vishwa Vidyapeeth Deemed to be University, Karad,
  5. Dr. Wasim Kamate , Department of Oral Pathology, School of Dental Sciences, Krishna Vishwa Vidyapeeth Deemed to be University, Karad,
  6. Dr. Rashmi Gangavati , Department of Oral Pathology, School of Dental Sciences, Krishna Vishwa Vidyapeeth Deemed to be University, Karad,

Abstract

Oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC) represent a spectrum of potentially malignant disorders posing significant challenges in diagnosis and management. The tumor microenvironment, particularly alterations in the connective tissue, plays a crucial role in the progression of these lesions. This histochemical investigation comprised with embedded polymers aimed to examine connective tissue changes across various grades of oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC). OED and OSCC represent a spectrum of potentially malignant disorders of the oral mucosa, with varying degrees of cellular and tissue alterations. However, the specific changes occurring in the connective tissue microenvironment across different grades of OED and OSCC remain poorly understood. In this study, histological sections from biopsied specimens of OED and OSCC were subjected to histochemical analysis to assess alterations in connective tissue components such as collagen fibers, mucins, and vascularization. Results revealed distinct patterns of connective tissue changes associated with different grades of OED and OSCC, highlighting the potential diagnostic and prognostic significance of these alterations in oral cancer progression through nanotechnological polymeric investigation.

Keywords

References (23)

  1. Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. International Journal of Cancer. 2010;127(12):2893-2917. doi:10.1002/ijc.25516
  2. Curado MP, Edwards B, Shin HR, Storm H, Ferlay J, Heanue M, et al. Cancer incidence in five continents, Volume IX. IARC Press, International Agency for Research on Cancer; 2007.
  3. George J, Narang RS, Rao NN. Stromal response in different histological grades of oral squamous cell carcinoma: A histochemical study. Indian Journal of Dental Research. 2012 Nov 1;23(6):842. Available from: https://www.ijdr.in/text.asp?2012/23/6/842/111291
  4. Acton AQ. Advances in Immune System Research and Applications. 1[sup]st ed. Atlanta, Georgia: Scholarly Editions; 2011. p. 1-3.
  5. Li H, Fan X, Houghton J. Tumor microenvironment: The role of the tumor stroma in cancer. Journal of Cellular Biochemistry. 2007;101(4):805-815. doi:10.1002/jcb.21159
  6. Sridhara SU, Choudaha N, Kasetty S, Joshi PS, Kallianpur S, Tijare M. Stromal myofibroblasts in nonmetastatic and metastatic oral squamous cell carcinoma: An immunohistochemical study. Journal of Oral and Maxillofacial Pathology: JOMFP. 2013 May;17(2):190. DOI:4103/0973-029X.119758
  7. Rao B, Malathi N, Narashiman S, Rajan ST. Evaluation of myofibroblasts by expression of alpha smooth muscle actin: a marker in fibrosis, dysplasia and carcinoma. Journal of clinical and diagnostic research: JCDR. 2014 Apr;8(4):ZC14. DOI: 7860/JCDR/2014/7820.4231
  8. Kalele KP, Managoli NA, Roopa NM, Kulkarni M, Bagul N, Kheur S. Assessment of collagen fiber nature, spatial distribution, hue and its correlation with invasion and metastasis in oral squamous cell carcinoma and surgical margins using Picro Sirius red and polarized microscope. Journal of Dental Research and Reviews. 2014;1(1):14-17. doi:10.4103/2348-3172.126159
  9. Fuentes B, Duaso J, Droguett D, Castillo C, Donoso W, Rivera C, et al. Progressive Extracellular Matrix Disorganization in Chemically Induced Murine Oral Squamous Cell Carcinoma. ISRN Pathology. 2012;2012:1-7. doi:10.5402/2012/359421
  10. Pereira AL, Veras SS, Silveira ÉJ, Seabra FR, Pinto LP, Souza LB, et al. The role of matrix extracellular proteins and metalloproteinases in head and neck carcinomas: an updated review. RevistaBrasileira de Otorrinolaringologia. 2005;71:81-6. Available from: https://doi.org/10.1590/S0034-72992005000100014
  11. Dvorak HF. Tumours: wounds that do not heal. New England Journal of Medicine. 1986 Dec 25;315(26):1650-9. DOI: 1056/NEJM198612253152606
  12. Reibel J, Gale N, Hille J, Hunt JL, Lingen M, Muller S, et al. Oral potentially malignant disorders and oral epithelial dysplasia. WHO classification of head and neck tumours. 2017;4:112-5.
  13. Jaber MA, Elameen EM. Long-term follow-up of oral epithelial dysplasia: A hospital based cross-sectional study. Journal of Dental Sciences. 2021;16(1):304-310. doi:10.1016/j.jds.2020.04.003
  14. Krolls SO, Hoffman S. Squamous cell carcinoma of the oral soft tissues: a statistical analysis of 14,253 cases by age, sex, and race of patients. The Journal of the American Dental Association. 1976;92(3):571-574. doi:10.14219/jada.archive.1976.0556
  15. Petti S. Lifestyle risk factors for oral cancer. Oral Oncology. 2009;45(4-5):340-350. doi:10.1016/j.oraloncology.2008.05.018
  16. Suvarna KS, Layton C, Bancroft JD, editors. Bancroft's theory and practice of histological techniques E-Book. Elsevier health sciences; 2018 Feb 27.
  17. Patankar SR, Wankhedkar DP, Tripathi NS, Bhatia SN, Sridharan G. Extracellular matrix in oral squamous cell carcinoma: Friend or foe?. Indian Journal of Dental Research. 2016 Mar 1;27(2):184. Available from: https://www.ijdr.in/text.asp?2016/27/2/184/183125
  18. Mehregan AH, Staricco RG. Elastic fibers in pigmented nevi. J Invest Dermatol. 1962 May 1;38:271-6.
  19. Mehregan AH, Staricco RG, Pinkus H. Elastic fibers in basal cell epithelioma. Archives of Dermatology. 1964 Jan 1;89(1):33-40.
  20. Elasbali AM, Al-Onzi Z, Hamza A, Khalafalla E, Ahmed HG. Morphological Patterns of Elastic and Reticulum Fibers in Breast Lesions. Health. 2018;10(12):1625-1633. doi:10.4236/health.2018.1012122
  21. Agrawal D, Zawar M, Deshpande N, Sudhamani S. The study of mucin histochemistry in benign and malignant lesions of prostate. Journal of the Scientific Society. 2014;41(1):38. doi:10.4103/0974-5009.126751
  22. Velidandla S, Gaikwad P, Ealla KK, Bhorgonde KD, Hunsingi P, Kumar A. Histochemical analysis of polarizing colors of collagen using Picrosirius Red staining in oral submucous fibrosis. Journal of international oral health: JIOH. 2014 Feb;6(1):33.
  23. Sharma R. Architectural Analysis of Picrosirius Red Stained Collagen in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma using Polarization Microscopy. JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH. 2015. doi:10.7860/jcdr/2015/13476.6872
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