Hexavalent Chromium Resistance Test of Saccharomyces Cerevisiae and Modeling of its Bioaccumulation Process

Tessafa abrham ashagrie; Shegaw Ahmed

Abstract

Now a day, the release of high chromium containing tannery waste water from tanning industries to the environment is the life threatening action of human activities and bioaccumulation becomes a better alternative in treating heavy metals(such as Cr(VI) )due to its cost effective, relatively high heavy metal removal capacity and recovery property. Cell suspensions of S.cerevisiae at its exponential growth phase were added to 250 ml plastic flasks of containing 50 ml of YPD media at pH value of (3, 5 & 8), initial Cr (VI) concentration of (5, 50 & 100 mg/ml) and incubation time of (3, 5 & 7 days) and shaken at 200 rpm and 27°C. Cell viability test assay to know the minimum inhibitory concentration and modeling of the bioaccumulation process to know dynamics is analyzed. Viability test of this work shows the possibility of Cr (VI) removal at its high concentration using living cells that are tolerant to relatively high concentration though the efficiency decreases as concentration increases due to inhibition and Equilibrium data fitted very well with Freundlic isotherm model and pseudo second order kinetic model are used for expressing the bioaccumulation dynamics of Cr(VI) based on their higher correlation coefficient (R2)value of 0.7804 and 0.99 respectively.

Abstract

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