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Cloning and Expression of LPS-Binding Single Domain Antibody Clones Selected from Phage Display Library of Indian Desert CamelBy Ajit Singh, Akhil Kumar Gupta
Abstract: Despite the absence of light chains in their structure, camelid and shark “heavy chain antibodies” (HCAbs) variable domains (designated as VHH) are able to make functional paratopes. The authors have selected lipopolysaccharide (LPS)-binder “single-domain antibodies” (dAb) clones from the previously constructed phage display library of (LPS)-immunized Indian desert camel. This study was undertaken to make available large amounts of some LPS-binder dAb clones by using a suitable expression vector-host system …
Published in Research and Reviews : A Journal of Immunology · Vol. 4, Issue 1, 2014 · pp. 1–7 Read article →