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Review on UV – Visible Spectroscopy and HPLC
Abstract: Like Fourier-transform infrared spectroscopy, UV-visible spectroscopy is used to determine the purity of pharmaceutical compounds. Many substances have chromospheres, which absorb certain wavelengths of ultraviolet or visible light. Using the Beer–Lambert equation, the absorption of spectra obtained from these samples at given wavelengths can be directly related to the sample concentration. Normally, UV and UV-visible spectra are recorded at high and low pH, and the results of both are compared …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 13, Issue 1, 2022 · pp. 43–54 Read article