aggregation propensity (SAP)

1 article · search the full text for this term

• Published Subscription Review Article 5 May 2026

  Integrative Structural-Functional Genomics of Fc and Fab: Precision Models for Monoclonal Antibody Stability and Anti-Aggregation Engineering

  By Tapaswi Ram Khajuria, Prof (Dr.) Atul Khajuria, KIRAN KUMARI

  Abstract: Monoclonal antibodies (mAbs) represent the cornerstone of biotherapeutics, yet aggregation propensity compromises up to 50% of candidates during development, driven by Fab hypervariability and Fc vulnerabilities.(1,2) This review integrates functional genomics from OAS (4B+ sequences)(5) and structural databases (SAbDab: 10K+ structures)(6) with machine learning models achieving R=0.97 for SAP prediction.(11) We dissect biophysical mechanisms, benchmark predictive tools (DeepSP, ESM2), and engineering strategies (YTE, FW mutations) that enhance Tm by 5-10°C …

  Published in International Journal of Molecular Biotechnological Research · Vol. 4, Issue 1, 2026 Read article →