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2 articles for “E. coli BL21 (DE3)”

• Published Subscription 4 Sep 2012

  Molecular Cloning, Expression, Purification, and Characterization of Methionine Aminopeptidase

  By Srinivasulu Yeruva, Muhammed Akbar, Priyanka Kola, Dindu Alekhya, Chinthala Hima Bindu, Subramanyam Mantha

  Abstract: MAP enzyme can efficiently remove N-terminal methionine from recombinant proteins. In the present study, MAP gene from genomic DNA of Escherichia coli was amplified, cloned into pET 11b vector and transformed into E.coli DH5a cloning strain to confirm the insertion of MAP gene, then plasmid was transformed into E. coli BL21 (DE3) expression strain. The protein was eluted with 25 mM NaCl and showed a purity of ~ 95% which …

  Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 2, Issue 2, 2012 · pp. 12–24 Read article →

• Published Subscription 3 Apr 2019

  Cloning and Expression of LPS-Binding Single Domain Antibody Clones Selected from Phage Display Library of Indian Desert Camel

  By Ajit Singh, Akhil Kumar Gupta

  Abstract: Despite the absence of light chains in their structure, camelid and shark “heavy chain antibodies” (HCAbs) variable domains (designated as VHH) are able to make functional paratopes. The authors have selected lipopolysaccharide (LPS)-binder “single-domain antibodies” (dAb) clones from the previously constructed phage display library of (LPS)-immunized Indian desert camel. This study was undertaken to make available large amounts of some LPS-binder dAb clones by using a suitable expression vector-host system …

  Published in Research and Reviews : A Journal of Immunology · Vol. 4, Issue 1, 2014 · pp. 1–7 Read article →