Search
247 articles for “assay”
-
Allelopathic Effect of Amaranthus Viridis.L Fresh Leaves on Chickpea Seeds Under in Vivo Condition
Abstract: Amaranthus viridis.L is a weed and its fresh leaves were collected from Charotar University of Science and technology campus. After cleaning the leaves were separated and taken for study purpose. One gram of fresh leaves was initially crushed to remove carotenoids. Then other one gram of fresh leaves was taken. Both types of fresh leaves along with control were considered for the experiment. Ten Chick pea (Cicer arientinum.L) seeds each …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 9, Issue 2, 2019 · pp. 26–29 Read article
-
A Method of Universal Primer Design for The Detection of Diverged CTX-M Beta-Lactamases in Multi-Drug Resistant Superbugs
Abstract: Complexity of many diverged β-lactamases in common multi-drug resistant bacteria has reached very high level. We found >40% of common bacteria in Kolkata water bodies were ampicillin resistant and reported clinical isolates were >95% ampicillin resistant worldwide. More than twenty unique sequence classes of β-lactamases were known to cause MDR inactivating penicillin, cephalosporin and carbapenem drugs. Further, there are other diverged MDR genes like drug efflux genes (acrAB, mexAB-EF, tetA), …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 9, Issue 2, 2019 · pp. 1–10 Read article
-
Evaluation of Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) Reagents on ECL 760 Coagulation Analyzer
Abstract: Prothrombin time (PT) and activated partial thromboplastin time (APTT) are two essential coagulation screening tests in various critical disorders including cardiovascular diseases and diabetes. Due to its criticality, accurate assessment of both the parameters are essential and fully automated coagulation analyzers like Erba ECL 760 can be of instrument of choice in this situation. The plasma collected from sodium citrated whole blood is preferred sample for coagulation analysis on fully …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 6, Issue 3, 2019 · pp. 5–11 Read article
-
Chemical Content Variations of Hypericum perforatum Growing in Turkey during its Ontogenetic and Diurnal Cycles
Abstract: AbstractThe biological activities of Hypericum perforatum have been mainly attributed to its hypericin and hyperforin contents. The present study was conducted to determine ontogenetic, morphogenetic and diurnal variation of hypericin, pseudohypericin and hyperforin contents in H. perforatum growing in Turkey. Plants were harvested at floral budding, full flowering, fresh fruiting and mature fruiting stages three times a day. When harvested they were dissected into stem, leaf and reproductive tissues, which …
Published in Research & Reviews : Journal of Botany · Vol. 5, Issue 1, 2016 · pp. 3–12 Read article
-
Efficiency of Waste Banana Peels in Bio-ethanol Production
Abstract: Aim: To check the efficiency of waste banana peels in bio-ethanol production. Methods and Results: Waste from banana fruit peel powder is subjected to the simultaneous saccharification and fermentation process for 7–10 days of co-culture of Aspergillus niger and Saccharomyces cerevisiae and also with a co-culture of Bacillus subtilis and Saccharomyces cerevisiae, in two different batch processes. The extracted β-amylase enzyme from the biomass of Aspergillus niger is also used …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 7, Issue 3, 2017 · pp. 28–32 Read article
-
Variation in Activity of Lipase Immobilized on Chitosan and Alginate Nanoparticles by Changing Concentration of the Preparatory Reagents
Abstract: Chitosan and alginate nanoparticles were prepared by ionic gelation method. The concentrations of each of the preparatory reagents (for chitosan: acetic acid, chitosan, sodium sulphate, glutaraldehyde and for alginate: calcium chloride, alginate, glutaraldehyde) were altered to check their consequences. Lipase was chemically crosslinked to these nanoparticles. Enzymatic assay was carried out for every alteration separately. To visualize the changes in each reagent at every step a precise table is incorporated …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 7, Issue 3, 2017 · pp. 14–22 Read article
-
Lasiosiphon glaucus: Preliminary Phytochemical Screening, Antioxidant Activity and Anti-inflammatory Activity of Ethnomedicinal Plant
Abstract: An important ethnobotanical medicinal plant Lasiosiphon glaucus was evaluated for its antioxidant and anti-inflammatory potentials. The plant showed significant antioxidant activity as evaluated by DPPH free radical scavenging activity (IC50 100 µg/ml) and ABTS radical scavenging activity (IC50 96 µg/ml) and Total antioxidant activity of 33 mg/g ascorbic acid equivalent. The plant has a total phenol content of 8.7 mg/g gallic acid equivalent. It is evident form HRBC membrane destabilisation …
Published in Research & Reviews : Journal of Herbal Science · Vol. 7, Issue 1, 2018 · pp. 1–7 Read article
-
In vitroAntioxidant and Antidiabetic Activity of Regional Chemotypes of three Curcuma species from Chhattisgarh
Abstract: Phytoconstituents and nutritional rich Curcuma species playsan important role in medication. In the present study rhizomes of Curcuma longa, Curcuma caesia and Curcuma aromatica from different regions of Chhattisgarh, Indiawere chosen for best chemotype identification on the basis of antioxidant and antidiabetic potential. Curcuminoids found in Curcuma rhizomes vary often with varieties, locations, sources, and cultivation conditions, while there are significant variations in varieties and geographical locations as well as …
Published in Research & Reviews : Journal of Herbal Science · Vol. 7, Issue 1, 2018 · pp. 13–22 Read article
-
Detection and Killing of Food Poisoning Salmonella Typhimurium in Cheese by Using Monoclonal Antibody and Nanoparticles Complex
Abstract: Present studies were conducted to test the ability of gold nanoparticles (GNPs) to detect and kill the bacterial contaminated food samples like cheese. The GNPs were prepared in two methods, sample A was purple 72 to 78.3 nm, while sample B was wine-red and the size was 51.5 to 58 nm. The results obtained suggest that the GNPs were synthesized with different particle size. UV-VIS spectroscopy and zeta potential tests …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 5, Issue 1, 2016 · pp. 1–10 Read article
-
Marine Algal Secondary Metabolites Promising Anti-Angiogenesis Factor against Retinal Neovascularization in CAM Model
Abstract: Retinal angiogenesis is an angle of new blood vessels on retinal surface. This neovascularization condition within the eye contributes to visual loss. Commonest cause of this condition includes diabetes, retinopathy of prematurity, retinal vein occlusion, etc. A variety of endothelial cell growth factors have been identified as a responsible factor and previous studies report that marine metabolites are promising molecules against retinal angiogenesis. Based on the background information collected, the …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 8, Issue 1, 2018 · pp. 19–25 Read article
-
Amentoflavone and Mangiferin in Hypericum calycinum, Hypericum cardiophyllum and Hypericum bithynicum
Abstract: The present study was conducted out to determine amentoflavone and mangiferin contents of Hypericum calycinum, Hypericum cardiophyllum and Hypericum bithynicum, growing wild in Turkey. The aerial parts representing a total of 30 individuals were collected at full flowering, dried at room temperature and assayed for secondary metabolite concentrations by HPLC-PDA. Both compounds were detected in all three species in different amounts depending on plant tissue. Results were discussed in phytochemical …
Published in Research & Reviews : Journal of Herbal Science · Vol. 6, Issue 1, 2017 · pp. 10–13 Read article
-
Phytochemicals, Antioxidant and Antidiabetic Properties of Nepata cateria and Arnebia benthamii of Kashmir (India)
Abstract: Nepata cateria and Arnebia benthamii two reputed folk medicinal plants used by Kashmiri people. Nepata cateria is commonly used in ointment as mosquito repellents. Both the plants show both antioxidant and antidiabetic properties in vivo as well as in vitro. Phytochemical test were done in order to screen the presence of terpenoids, flavonoids, saponins, tannins, polyphenols, alkaloids, anthraquinones and cardiac glycosides and all the result were positive. As well as …
Published in Research & Reviews : Journal of Herbal Science · Vol. 5, Issue 2, 2016 · pp. 6–13 Read article
-
Evaluation of Antimicrobial and -Amylase Inhibitory Activity of Selected Medicinal Plants
Abstract: During this study we evaluated the a-amylase inhibitory activity and antimicrobial activity of different extracts of Psidium gujauva, Allium sativum, Withania sominfera, and Piper nigrum. Antimicrobial potential was observed against Escherichia coli and Staphylococcus aureus. The plant extracts were prepared with chloroform, ethanol, acetone and distilled water. Each extract was evaporated using rotary evaporator, under reduced pressure. Different concentrations (10, 20, 40, 60, 80, and 100 μg/ml) of each extract …
Published in Research & Reviews : Journal of Herbal Science · Vol. 4, Issue 1, 2015 · pp. 15–28 Read article
-
Nitric-oxide Scavenging Antioxidant Activity of the Different Extracts of Plumeria obtusa (Family:Apocynaceae)
Abstract: Plumeria obtusa (Family-Apocynaceae) has a long ethnomedicinal tradition of being used as a remedial agent in various diseases such as diarrhoea, piles, fever, gonorrhoea, antibacterial and antitumour agents etc. Antioxidant potential of the crude petroleum ether, chloroform and methanol extracts of the leaf and bark were evaluated by using nitric oxide scavenging assay. The extracts showed significant activities compared to ascorbic acid in a dose dependent mode. Moreover, the percentage …
Published in Research & Reviews : Journal of Herbal Science · Vol. 4, Issue 1, 2015 · pp. 58–62 Read article
-
Comparative Exploration of Medicinal and Photosynthetic Occurrences of Coriandrum sativum, Menthapiperita and Spinacia oleracea
Abstract: AbstractPlants form a large group of valuable materials like medicines, alkaloids, flavonoids, carotenes, and chlorophylls. To evaluate these activities leaves of three plant samples i.e., Coriandrum sativum, Mentha piperita and Spinacia oleracea were used in this study and two organic solvents i.e., acetone and ethanol were used to extract the antibacterials and antioxidants from plant samples. Total flavonoid content, total phenolic content and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging methods were …
Published in Research & Reviews : Journal of Herbal Science · Vol. 2, Issue 3, 2013 · pp. 15–23 Read article
-
Evaluation of Different Essential Oils for Antifungal Activity on Postharvest Decay of Avocado (Persea Americana Mill.) of Sikkim, India
Abstract: Commercially available essential oils of Basil oil, Clove oil, Citronella oil, Ginger oil and methanol extracted fern oil, Artemisia oil, and Stinging nettle oil were tested for growth inhibitions of two fungi isolated from infected avocado fruit from Sikkim, India which were identified as Fusarium sp. and Penicillium digitatum on the basis of colony morphology and microscopic examination. Inhibitory effect of essential oils was investigated by well diffusion method by …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 2, 2019 · pp. 44–51 Read article
-
Bacteriocin Production and Characterization Obtained from Lactobacillus sakei
Abstract: Natural preservatives such as bacteriocins become an attractive alternative in the substitution of chemical preservatives, which have eventual potential for toxicity and carcinogenic effect. In this study, a bacteriocin was produced from a strain of Lactobacillus sakei, isolated from Italian salami. The data obtained in this paper revealed that the antimicrobial activity of bacteriocin was higher than 85% in the assays using the Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 10, Issue 1, 2021 · pp. 27–36 Read article
-
Isolation, Characterization and Molecular Identification of Potential Probiotic Bacterium, Bacillus amyloliquefaciens CS4 from gut of Snakehead Fish, Channa striata (Bloch, 1793)
Abstract: The present study was to isolate and characterize the potential probiotic bacterium from the gutof healthy fish, Channa striata. Totally, 37 bacteria were isolated from the gut region, in whichonly 25 isolates that showed catalase negative were selected. Among the selected isolates, CS4exhibited maximum inhibitory activity against fish (Aeromonas hydrophila, Vibrio harveyi,Vibrio parahaemolyticus) and human (Serratia marcescens) pathogenic bacteria. Theisolate CS4 exhibited potential tolerance to 0.5% bile salts at 24 …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 2, 2019 · pp. 1–10 Read article
-
Identification and Quantification of Anti-inflammatory Compound in Michelia champaca
Abstract: Ferulic acid is a ubiquitous phenolic acid present in the plants. This phenolic acid is of low toxicity and can easily be absorbed and metabolized in the human body. Ferulic acid has many physiological functions such as anti-inflammatory, antioxidant, antimicrobial, anti-thrombotic and anti-cancerous activities. In the present study, Ferulic acid was isolated from Michelia champaca extract and quantified through High Performance Liquid Chromatography (HPLC) analysis. Ferulic acid is effective in …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 1, 2019 · pp. 16–23 Read article
-
Investigation of Annonaceae Acetogenins for Inhibition of Dengue Viral Protease using in silico Docking Studies
Abstract: Dengue viruses (DENVs) cause severe and widespread epidemics of diseases such as dengue fever and dengue hemorrhagic fever/dengue shock syndrome. Unfortunately, there is at present neither prophylaxis nor specific treatment to cure the disease and thus it is need of the hour to develop new antiviral therapeutic agents to combat diseases caused by DENV. Non-structural protein NS3 play a major role in the processing of polyprotein and replication of the …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 1, 2019 · pp. 47–52 Read article