STM Journals Peer-reviewed research from Consortium e-Learning Network

Research and Reviews : A Journal of Life Sciences Article Open Access

Characterization of Plant Growth Promoting Bacillus subtilis (VBKT5) Isolated from Vermicompost

  1. Sucharita Ghosh Parasitology and Microbiology Research Laboratory, Department of Zoology, The University of Burdwan
  2. Tilak Nayak Department of Life Science and Biotechnology, Jadavpur University
  3. Paltu Kumar Dhal
  4. Tushar Kanti Dangar
  5. Soumendranath Chatterjee

Abstract

Vermicompost is a rich source of nutrients and beneficial soil bacteria which can promote plant growth in various ways. Ten prevalent bacterial strains were isolated from the cow dung vermicompost and assessed for growth promotion of paddy. Bacterial suspensions (3X108 CFU/ml) were individually applied to the pots planted with paddy variety and vegetative growth parameters and yield were recorded. Among the bacterial treatments, better shoot length, root length and yield were recorded for Bacillus subtilis (VBKT5). Average yield in untreated control plants was less than a half of the yield for B. subtilis (VBKT5) treated plant. This strain was Gram positive, rod shaped, endospore forming bacillus and identified as B. subtilis. The results of the present study clearly indicated that B. subtilis isolated from vermicompost significantly boosted up growth and production of paddy plants and the strain may be explored as a plant growth promoting bacteria (PGPB) for rice improvement.

Keywords

References (30)

  1. Barman M, Paul S, Choudhury AG, Roy P, Sen J. Biofertilizer as Prospective Input for Sustainable Agriculture in India. International Journal of Current Microbiology and Applied Sciences. 2017;6(11):1177-1186. doi:10.20546/ijcmas.2017.611.141
  2. Roychowdhury D, Mondal S, Banerjee SK. The Effect of Biofertilizers and the Effect of Vermicompost on the Cultivation and Productivity of Maize - A Review. Advances in Crop Science and Technology. 2017;05(01). doi:10.4172/2329-8863.1000261
  3. Hashemimajd K, Kalbasi M, Golchin A, Shariatmadari H. Comparison of Vermicompost and Composts as Potting Media for Growth of Tomatoes. Journal of Plant Nutrition. 2004;27(6):1107-1123. doi:10.1081/pln-120037538
  4. Sinha RK, Agarwal S, Chauhan K, Valani D. The wonders of earthworms & its vermicompost in farm production: Charles Darwin’s ‘friends of farmers’, with potential to replace destructive chemical fertilizers. Agricultural Sciences. 2010;01(02):76-94. doi:10.4236/as.2010.12011
  5. Vennila C, Jayanthi C, Sankaran VM. Vermicompost on crop production—a review. Agric Rev. 2012; 33(3): 265–270p.
  6. Munnoli, PM, Silva JAT, Saroj B. Dynamics of the soil-earthworm-plant relationship: A review. Dyn Soil Dyn Plant. 2010; 4: 1–21p.
  7. Mohammad Taghi Darzi. Effects of the application of organic manure and biofertilizer on the fruit yield and yield components in Dill (Anethum graveolens). Journal of Medicinal Plants Research. 2012;6(17). doi:10.5897/jmpr12.204
  8. Matteoli FP, Passarelli-Araujo H, Reis RJA, da Rocha LO, de Souza EM, Aravind L, et al. Genome sequencing and assessment of plant growth-promoting properties of a Serratia marcescens strain isolated from vermicompost. BMC Genomics. 2018;19(1). doi:10.1186/s12864-018-5130-y
  9. Sustainable Utilization of Natural Resources. 2017. doi:10.1201/9781315153292
  10. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey's Manual of Determinative Bacteriology, 9th Edn. Baltimore: The Williams and Wilkins Co.; 1994.
  11. Smibert RM, Krieg NR. Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR, editors. Methods of general and molecular bacteriology. Washington DC: American Society for Microbiology; 1994.
  12. Logan NS, Vos P, Genus I. Bacillus. In: de Vos P, Garrity GM, Jones D, Krieg NR, Ludwing W, Rainey FA, Schleifer KH, Whitman WB, editors. Bergey’s manual of systematic bacteriology, 3(2). New York: The Firmicutes Springer; 2009.
  13. Cappuccino JG, Sherman N. Biochemical activities of microorganisms. In: Microbiology, a laboratory manual; 10th Edn. New Delhi, India: Pearson; 2014.
  14. Brown A, Smith H. Benson’s Microbiological Applications, Laboratory Manual in General Microbiology, Short Version. USA: McGraw-Hill Education; 2014.
  15. Krumpernam PH. Multiple antibiotic resistance indexing Escherichia coli to identify risk sources of faecal contamination of foods. Appl Environ Microbiol. 1983; 46: 165–170p.
  16. Douglas A, Atchison B. Degradation of DNA during the denaturation step of PCR. PCR Methods Appl. 1993; 3: 133–134p.
  17. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research. 1994;22(22):4673-4680. doi:10.1093/nar/22.22.4673
  18. Jukes TH, Cantor CR. Evolution of protein molecules. In: Munro HN, editor. Mammalian Protein Metabolism. New York: Academic Press; 1969.
  19. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution. 2007;24(8):1596-1599. doi:10.1093/molbev/msm092
  20. Darzi MT, Ghalavand A, Rejali F. Effect of mycorrhiza, vermicompost and phosphate biofertilizer application on flowering, biological yield and root colonization in fennel (Foeniculum vulgare Mill.). Iran J Crop Sci. 2008; 10(1): 88–109p.
  21. Ratti N, Kumar S, Verma HN, Gautam SP. Improvement in bioavailability of tricalcium phosphate to Cymbopogon martinii var. motia by rhizobacteria, AMF and Azospirillum inoculation. Microbiological Research. 2001;156(2):145-149. doi:10.1078/0944-5013-00095
  22. Shaalan MN. Effect of compost and different sources of biofertilizers, on borage plants (Borago officinalis L.). Egypt J Agric Res. 2005a; 83(1): 271–284p.
  23. Shaalan MN. Influence of biofertilizers and chicken manure on growth, yield and seeds quality of (Nigella sativa, L.) plants. Egypt J Agric Res. 2005b; 83(2): 811–828p.
  24. Rashmi KR, Earanna N, Vasundhara M. Influence of biofertilizers on growth, biomass and biochemical constituents of Ocimum gratissimum. L. Biomed. 2008; 3(2): 123–130p.
  25. Singh S, Kapoor KK. Effects of inoculation of phosphate-solubilizing microorganisms and an arbuscular mycorrhizal fungus on mungbean grown under natural soil conditions. Mycorrhiza. 1998;7(5):249-253. doi:10.1007/s005720050188
  26. de Boer Sietske A, Diderichsen B. On the safety of Bacillus subtilis and B. amyloliquefaciens: a review. Applied Microbiology and Biotechnology. 1991;36(1):1-4. doi:10.1007/bf00164689
  27. Vaz-Moreira I, Faria C, Lopes AR, Svensson L, Falsen E, Moore ER, Ferreira ACS, Nunes OC, Manaia CM. Sphingobium vermicomposti sp. nov., isolated from vermicompost. Int J Syst Evol Microbiol. 2009; 59(12): 3145–3149p. Available from: https://doi.org/10.1099 /ijs.0.006163-0.
  28. Kohler J, Caravaca F, Carrasco L, Roldán A. Interactions between a plant growth-promoting rhizobacterium, an AM fungus and a phosphate-solubilising fungus in the rhizosphere of Lactuca sativa. Applied Soil Ecology. 2007;35(3):480-487. doi:10.1016/j.apsoil.2006.10.006
  29. Pandya U, Prakash S, Shende K, Dhuldhaj U, Saraf M. Multifarious allelochemicals exhibiting antifungal activity from Bacillus subtilis MBCU5. 3 Biotech. 2017;7(3). doi:10.1007/s13205-017-0827-1
  30. Singh N, Pandey P, Dubey RC, Maheshwari DK. Biological control of root rot fungus Macrophomina phaseolina and growth enhancement of Pinus roxburghii (Sarg.) by rhizosphere competent Bacillus subtilis BN1. World Journal of Microbiology and Biotechnology. 2008;24(9):1669-1679. doi:10.1007/s11274-008-9680-z
Support