STM Journals Peer-reviewed research from Consortium e-Learning Network

Research and Reviews : Journal of Crop science and Technology

Effect of Different Priming Methods on Germination Behaviour of Rice in Drought Environment

  1. Bipin Aryal
  2. Roshan Subedi
  3. Narayan Neupane

Abstract

Drought is a major abiotic stress that hinders germination and seedling growth in rice. Proper priming of seeds prior to sowing has been a well-established technique to improve germination and seedling growth under drought. An experiment was conducted in Agronomy lab of Lamjung campus to study the effectiveness of different priming methods in improving germination of rice under water stress. Experiment was laid out in Complete Randomized Design with 10 treatments each replicated three times. Treatments used were hydropriming, halopriming (NaCl @ 1% and 2%), osmopriming (PEG-600 @ 1%, 2%, 5% and 10%), hormonal priming (GA3 @ 2.5 ppm and 5 ppm) and control (without priming). Osmotic potential of -1 bars was created using PEG-4000. Result showed that hydropriming significantly reduced mean germination time but did not subsequently enhance other germination parameters and total germination than unprimed seeds. NaCl improved the overall germination percentage but not the speed of germination and proved to be effective particularly at lower concentration of 1%. In contrast increasing concentrations of GA3 resulted in better germination and seedling vigor indices. Priming rice seeds with PEG were found more effective in improving germination, seedling vigor and dry matter accumulation. In all the parameters, the effect of priming with PEG improved with increasing concentration from 1% to 10%. The results concluded that priming with PEG and GA3 at higher concentration would be beneficial in improving germination behavior of rice seeds in a comparatively drier condition. Keywords: Drought stress, germination, halopriming, hydropriming, PEGCite this ArticleAryal B, Subedi R, Neupane N. Effect of Different Priming Methods on Germination Behaviour of Rice in Drought Environment. Research & Reviews: Journal of Crop Science and Technology. 2018; 7(2): 22–28p

References (27)

  1. Mahajan S , Tuteja N. Cold, salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics . 2005; 444(2): 139–58p.
  2. Gauchan D, Pant HK, Gautam S, et al. Patterns of adoption of improved rice varieties and farm level impact in stress prone rainfed areas of Nepal. In: Patterns of adoption of improved rice varieties and farm-level impacts in stress -prone rainfed areas in South Asia . Los Baños, Philippines: International Rice Research Institute (IRRI); 2012. 318p.
  3. Manikavelu A, Nadarajan N, Ganesh SK, et al. Drought tolerance in rice: morphological and molecular genetic consideration. Plant Growth Regul . 2006; 50: 121–38p. 10.1007/s10725-006-9109-3
  4. Bewley JD, Black M . Physiology and biochemistry of seeds in relation to germination. New York: Springer-Verlag; 1982.
  5. Basra SMA, Farooq M, Khaliq A. Comparative study of pre -sowing seed enhancement treatments in indica rice (Oryza sativa L.). Pakistan Journal of Life and Social Science. 2003; 1: 5–9p.
  6. Ashraf M , Foolad MR. Pre -sowing seed treatment – a shotgun approach to improve germination, plant growth, and crop yield under saline and non -saline conditions. Adv Agron. 2005; 88: 223–71p.
  7. Yuan-Yuan S, Yong-Jian S, Ming-Tian W, et al. Effects of Seed Priming on Germination and Seedling Growth Under Water Stress in Rice. Acta Agronomica Sinica. 2010; 36: 1931–40p. 10.1016/S1875-2780(09)60085-7.
  8. Wang X, Shen B . Effect of MET -soaked seed on drought resistance of rice seedlings. Acta Phytophysiol Sin . 1991; 17: 105–8p.
  9. Global Rice Science Partnership (GRiSP). Rice almanac, 4th Edn. Los Baños: International Rice Research Institute ; 2013.
  10. Bam RK, Kumaga FK, Ori K , et al . Germination, vigour and dehydrogenase activity of naturally aged rice ( Oryza sativa L.) seeds soaked in potassium and phosphorus. Asian Journal of Plant Sciences. 2006; 5(6): 948–55p.
  11. Orchard T. Estimating the parameters of plant seedling emergence. Seed Science and Technology. 1977; 5: 61–9p.
  12. Abdul-Baki AA , Anderson JD. Vigor determination in soybean seed by multiple criteria. Crop Sci. 1973; 13: 630–3p
  13. Ajouri A, Haben A, Becker M. Seed priming enhances germination and seedling growth of barley under conditions of P and Zn deficiency. J Plant Nut Soil Sci. 2004; 167: 630–6p.
  14. Asgedom H, Becker M. Effects of seed priming with nutrient solutions on germination, seedling growth and weed competitiveness of cereals in Eritrea. In: Proc. Deutscher Tropentag, University of Bonn and ATSAF. Weickersheim: Magrraf Publishers Press; 2001. 282p.
  15. Rowse HR. Drum Priming- A non-osmotic method of priming seeds. Seed Sci Technol. 1995; 24: 281–94p.
  16. Chen K, Arora R, Arora U. Osmopriming of spinach ( Spinaca oleracea ) seeds and germination performance under temperature and water stress. Seed Sci Technol. 2010; 38: 45–57p.
  17. Baque A, Nahar M, Yeasmin M, et al . Germination Behavior of Wheat ( Triticum aestivum L.) as Influenced by Polyethylene Glycol (PEG). Universal Journal of Agricultural Research . 2016; 4(3): 86–91p.
  18. Hus JL , Sung JM. Antioxidant role of glutatnione associated with accelerated agina and hydration of triploid Warermelon seeds. Physiologa Plantarum. 1997; 100: 967–74p. Research & Reviews: Journal of Crop Science and Technology Volume 7, Issue 2 ISSN: 2319-3395 (Online) RRJoCST (2018) 22-28 © STM Journals 2018. All Rights Reserved Page 28
  19. Esmaielpour B, Ghassemi -Golezani K, Khoei F, et al . The effect of seed treatments on vigour and yield of sunflower. Indian Journal of Plant Physiology. 1995; 38: 66–8p.
  20. Khajeh-Hosseini M, Powell AA, Bingham IJ. The interaction between salinity stress and seed vigor during germination of soybean seeds. Seed Sci Technol. 2003; 31:715–725p.
  21. Liptay A , Zariffa N. Testing the morphological aspects of polyethylene glycol primed tomato seeds with proportional odds analysis. Hort Sci. 1993; 28: 881–3p.
  22. Tsegay A, Berhanu, Gebremedhin Y. The role of seed priming in improving seedling growth of maize ( Zea mays L.) under salt stress at field conditions. Agricultural Sciences. 2013; 4: 666–72p.
  23. Price AH, Tomos AD, Virk DS. Genetic dissection of root growth in rice ( Oryza sativa L.). I: A hydroponic scre en. Theor & Appl Genet. 1997; 95: 132–42p.
  24. Ruan S, Xue Q, Tylkowska K. Effects of seed priming on germination and health of rice ( Oryza sativa L.) seeds. Seed Sci Technol. 2002; 30: 451–8p.
  25. GongPing GU, GuoRong WU, ChangMei L, et al . Effects of PEGprimng on Vigor index and activated oxygen metabolism in soyabean seedlings. Chinese Journal of Oil Crop Science. 2000; 1: 13–31p.
  26. Farooq M, Basra S, Saleem B, et al . Enhancement of tomato seed germination and seedling vigor by osmopriming. Pakistan J Agric Sci. 2005; 42: 52–8p.
  27. Farooq M, Basra SMA, Rehman H, et al. Pre-sowing salicylicate seed treatments improve the germination and early seedling growth in fine rice. Pakistan J Agri Sci. 2007; 44: 16–23p. Cite this Article Aryal B, Subedi R , Neupane N. Effect of Different Priming Methods on Germination Behaviour of Rice in Drought Environment. Research & Reviews: Journal of Crop Science and Technology . 2018; 7(2): 22–28p.

You might also find these relevant

Support