Nanotechnology plays an important role in the development of new class of biosensors known as nanobiosensors. The performance and sensitivity of biosensors are improved by using nanomaterials through new signal transduction technologies. The design of biosensors has greatly witnessed significant alterations in the recent days. Firstly; biosensor is a device which converts biological responses as diverse range of applications in many fields like food quality estimation, environmental monitoring, clinical applications, diagnostic applications etc. Nanobiosensors commonly comprise of biological recognition molecule immobilized onto the surface of a signal transducer. The development of process and tools used to fabricate and image nanoscale objects has led the interaction of sensors with extremely small molecules which need to be analysed. Nanotechnology has best exciting ingredients for the improvement of sensing phenomenon. The use of wide varieties of nanomaterials ranging from nanoparticles, nanotubes, nanorods, and nanowires has drastically boosted the specificity of the detection system, and its reproducibility in much better way. This paper reviews the various nanostructure based biosensors and their applications. Keywords: Nanotechnology, nanobiosensors, biological recognition Cite this Article Abhishek B, Athiq M, Saranya D. A Review on Nano Biosensors and its Applications. Nanotrends: A Journal of Nanotechnology and its Applications. 2016; 18(1): 27–34p.