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Journal of Petroleum Engineering & Technology Original Research

Experimental Validation of a pH-Dependent Kinetic Model for Bioremediation of Crude Oil-Contaminated Soil Using Pseudomonas sp.

  1. G.A.O. Ajoku Lecturer I, Department of Chemical Engineering, University of Port Harcourt, Port Harcourt, Nigeria
  2. M.K. Oduola ✉ Professor, Department of Chemical Engineering, University of Port Harcourt, Port Harcourt, Nigeria

Abstract

A kinetic model that relates the pH effect on bioremediation of crude petroleum contaminated soil has been developed. The model depicts the dependence of enzyme-catalyzed reaction rates on pH and was derived at both mathematical and kinetic levels, with the resulting rate expressions derived as modifications of the Michaelis-Menten equation. The developed equations enables the calculation of equilibrium constants K1, K2, the specific growth rates Vi, as a function of change in pH of the medium, and the optimum hydrogen ion concentration H_opt^+ or (pH)_optof the process. In this work, a study on the biodegradation activities of a hydrocarbon utilizing bacterial, Pseudomonas sp, on a soil contaminated with crude petroleum was carried out to validate the developed model under variable pH conditions (4, 5, 6, 7, 8, and 9). The data obtained from experimental study were compared with data generated using the developed kinetic model. Reaction rates obtained from experiment at pH of 4, 5, 6, 7, 8, and 9 are 5.40E+04, 3.46+E05, 6.80+E05, 1.95E+06, 3.56E+06 and 6.87e+05 respectively while the rates of enzyme-catalyzed reaction obtained using the developed kinetic model and at the same pH conditions are 4.98E+04, 3.16E+05, 6.25E+05, 1.41E+06, 3.33E+06, and 5.77E+05 respectively.

Keywords

References (43)

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