Mobile ad-hoc networks (MANETs) are collection of wireless mobile computers (or nodes) having no pre-existing infrastructure or centralized management and which are connected by wireless links automatically. They are one of the most commonly used network services these days. They play a great role in tactical situations such as disaster relief or battlefields, making it an important area for research. But, unfortunately, the many factors that make MANETs successful (fluidity, the ability of a substance, and process of redistributing or dispersing functions) pose tremendous challenges for those tasked with securing those environments. Various types of attacks are possible on MANETs. Sleep deprivation attack is one of them. Securing mobile ad-hoc network is essential for network communications. Success of mobile networks (MANET) strongly depends on people’s confidence in its security. Sleep deprivation attack is a kind of DoS attack which can be set in motion by flooding the targeted node with not needed routing packets. In this paper, we have to show the presence of the sleep deprivation attack and their effect in one of the most prominent AODV (ad-hoc on an insistent and peremptory request distance vector) routing protocol. The proposed method works for comparing the effect of with and without sleep deprivation attack in AODV for parameter throughput. Keywords: Mobile ad hoc network, AODV protocol, sleep deprivation attackCite this Article Navneet Sandhu, Meenu Talwar. A Comparative Analysis of Sleep Deprivation Attack in AODV. Journal of Network Security. 2015; 3(3): 33–38p.