Preliminary Phytochemical Screening, Antioxidant and antibacterial activity of Helicteres isora leaf extracts.

V. Rajashakar; K. Mallikarjun; G. Ujwalasandya

Abstract

Objective: To evaluate phytochemical screening, Anti-oxidant and anti-bacterial activity of Helicteres isora leaf extracts. Methods: Petroleum ether, chloroform, acetone, ethanol and hydroalcoholic extracts of leaves were used for the phytochemical screening. Anti-oxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (No) radical scavenging tests; antibacterial activity was assessed using disc diffusion techniques. Results: The percentage yields for the petroleum ether, chloroform, acetone, ethanol and the hydroalcoholic extract were 1.29%, 1.76%, 1.41%, 2.18% and 3.23% w/w, respectively. H. isora leaf extract produced favourable results for sterols and flavonoids during the initial phytochemical screening. The DPPH radical-scavenging activity of the concentrations of ethanolic and hydroalcoholic extracts, and L ascorbic acid needed for 50% inhibition free radicals were found to be 84.02 µg/mL, 76.05 µg/mL and 54.96 µg/ml, respectively. The inhibition of No radicals generated from sodium nitroprusside at physiological pH by the ethanolic and hydroalcoholic extracts and L-ascorbic acid was observed at concentrations of 85.05 µg/mL, 74.03 µg/mL, and 51.69 µg/mL, respectively. Conclusions: The hydroalcoholic extract's observed antibacterial and antioxidant properties may be due to the phytochemical components of these substances