Utilization of the Sibininto treat the DMBA Induced Hepatotoxicity and Free-Radical Damage in Norwegian Rat, Rattus norvegicus (L).

Vitthalrao Bhimasha Khyade; Pragati Pramod Shinde; Aarti Sanjay Dhumal

Abstract

DMBA is abbreviated form of the 7,12-Dimethylbenz[a]anthracene. It is working as immuno-suppressor. It also serves as a tumor initiator. It allows for a greatly accelerated rate of growth of tumor and making many cancer studies possible. The plant, Silybum marianum (L) yields pharmacologically active compound named as silbinin. The silbinin deserve documented antioxidant activity. The purpose of this study was to assess this silbinin for protective influence in DMBA induced liver damage. The experimental animals (Norwegian Rat, Rattus norvegicus L) were divided into four groups, like control group (received oral corn oil ); DMBA group (received oral at the rate of 335 mg/kg in the corn oil solution); Silibinin group (received 100 mg/kg/day silibinin alone orally, every 24 hours for 7 days) and “Silibinin + DMBA” group (received DMBA plus silibinin). All animals were sacrificed at the end of attempt of schedule of treatment followed by rearing. The tissues used for biochemical assessment were blood and liver. The blood serum was assessed for the parameters that include: Superoxide dismutases (SOD), glutathione peroxidase (GPX), nitric oxide (NO) and myeloperoxidase (MPO) were investigated in serum and liver tissue. In addition, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzyme activities. The homogenate of liver tissue was evaluated histopathologically with Hematoxilin & Eosin dye. The ALT, AST, NO, MPO in serum and NO, MPO in liver tissue were found to be significantly higher in DIMBA treated group in comparison with control group (P < 0.001). In Group: Silibinin + DMBA, serum AST, ALT, NO, MPO levels were significantly lower (P < 0.01), and both serum and tissue SOD activities were significantly higher, compared to DMBA group (P < 0.001). DMBA induced histopathological changes in liver tissue include : severe sinusoidal dilatation, moderate disruption of the radial alignment of hepatocytes around the central vein, severe vacuolization in the hepatocyte cytoplasm, inflammation around central vein and portal region. In rats receiving both DMBA and silibinin, the DMBA induced changes accounted for less sinusoidal dilatation, vacuolization in the hepatocyte cytoplasm and the inflammation around central vein and portal region (P < 0.05). Silibinin exert reduction in the oxidative stress through inducing antioxidant mechanisms, there after showing protective effect against DMBA induced hepatic damage.

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