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International Journal of Genetic Modifications and Recombinations Review Article

Gene Editing in Dairy Cows: Advancing Milk Yield, Health, and Resilience for Sustainable Dairy Development

  1. Md. Emran Hossain Department of Animal Science and Nutrition, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram-4225
  2. Shilpi Islam Department of Animal Science and Nutrition, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706

Abstract

Gene editing has emerged as a transformative tool in dairy science, offering unprecedented opportunities to enhance productivity, improve animal health, and bolster resilience against environmental challenges. This study explores the potential of gene editing technologies, including CRISPR-Cas9, in advancing milk yield, optimizing milk composition, and addressing genetic disorders in dairy cows. Specific applications include targeting genes associated with mastitis resistance, improving feed efficiency, and introducing heat tolerance traits to adapt to climate variability. Additionally, gene editing provides solutions to reduce greenhouse gas emissions by modifying rumen microbiota, contributing to environmentally sustainable dairy farming practices. Ethical considerations and regulatory frameworks are also highlighted, addressing public concerns about animal welfare and food safety. This paper emphasizes the integration of gene editing into breeding programs to ensure precise, heritable trait modifications while minimizing off-target effects. By focusing on these advancements, gene editing can revolutionize dairy farming, enabling sustainable dairy development that balances productivity, animal well-being, and environmental stewardship. However, challenges such as societal acceptance, cost-effectiveness, and long-term impacts warrant further investigation. The findings underscore the critical role of gene editing in shaping the future of dairy production systems, offering a pathway toward sustainable and efficient dairy farming practices.

Keywords

References (76)

  1. Karavolias NG, Horner W, Abugu MN, Evanega SN. Application of Gene Editing for Climate Change in Agriculture. Frontiers in Sustainable Food Systems. 2021;5. doi:10.3389/fsufs.2021.685801
  2. Pickering NK, Oddy VH, Basarab J, Cammack K, Hayes B, Hegarty RS, et al. Animal board invited review: genetic possibilities to reduce enteric methane emissions from ruminants. Animal. 2015;9(9):1431-1440. doi:10.1017/s1751731115000968
  3. Mueller ML, Van Eenennaam AL. Synergistic power of genomic selection, assisted reproductive technologies, and gene editing to drive genetic improvement of cattle. CABI Agriculture and Bioscience. 2022;3(1). doi:10.1186/s43170-022-00080-z
  4. Van Eenennaam AL, Young AE. Gene editing in livestock: promise, prospects and policy. CABI Reviews. 2019:1-14. doi:10.1079/pavsnnr201813027
  5. Mishu M, Nath S, Sohidullah M, Hossain M. Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology. Journal of Advanced Veterinary and Animal Research. 2024;11(2):483. doi:10.5455/javar.2024.k798
  6. Moran MN, Jones DB, Jensen SA, Marcoli R, Jerry DR. Optimising commercial traits through gene editing in aquaculture: Strategies for accelerating genetic improvement. Reviews in Aquaculture. 2023;16(3):1127-1159. doi:10.1111/raq.12889
  7. Pozzebon M, Guldbrandtsen B, Sandøe P. Gene Editing Cattle for Enhancing Heat Tolerance: A Welfare Review of the “PRLR-SLICK Cattle” Case. NanoEthics. 2024;18(2). doi:10.1007/s11569-024-00455-8
  8. Yunes MC, Osório-Santos Z, von Keyserlingk MAG, Hötzel MJ. Gene Editing for Improved Animal Welfare and Production Traits in Cattle: Will This Technology Be Embraced or Rejected by the Public? Sustainability. 2021;13(9):4966. doi:10.3390/su13094966
  9. Yuan YG, Liu SZ, Farhab M, Lv MY, Zhang T, Cao SX. Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock. Functional & Integrative Genomics. 2024;24(3). doi:10.1007/s10142-024-01364-5
  10. Yunes MC, Osório-Santos Z, von Keyserlingk MAG, Hötzel MJ. Gene Editing for Improved Animal Welfare and Production Traits in Cattle: Will This Technology Be Embraced or Rejected by the Public? Sustainability. 2021;13(9):4966. doi:10.3390/su13094966
  11. Subedi U, Kader K, Jayawardhane KN, Poudel H, Chen G, Acharya S, et al. The Potential of Novel Gene Editing-Based Approaches in Forages and Rumen Archaea for Reducing Livestock Methane Emissions. Agriculture. 2022;12(11):1780. doi:10.3390/agriculture12111780
  12. Ngeno K. Utilization of Genome Editing for Livestock Resilience in Changing Environment. Black Sea Journal of Agriculture. 2023;6(3):314-320. doi:10.47115/bsagriculture.1263027
  13. Camargo LSA, Saraiva NZ, Oliveira CS, Carmickle A, Lemos DR, Siqueira LGB, et al. Perspectives of gene editing for cattle farming in tropical and subtropical regions. Animal Reproduction. 2022;19(4). doi:10.1590/1984-3143-ar2022-0108
  14. Söllner JH, Mettenleiter TC, Petersen B. Genome Editing Strategies to Protect Livestock from Viral Infections. Viruses. 2021;13(10):1996. doi:10.3390/v13101996
  15. University of California-Davis, USA, Van Eenennaam AL, Young AE, University of California-Davis, USA. The use of gene editing techniques in dairy cattle breeding. Burleigh Dodds Series in Agricultural Science. 2019:571-602. doi:10.19103/as.2019.0058.27
  16. Hansen PJ. Prospects for gene introgression or gene editing as a strategy for reduction of the impact of heat stress on production and reproduction in cattle. Theriogenology. 2020;154:190-202. doi:10.1016/j.theriogenology.2020.05.010
  17. Gutási A, Hammer SE, El-Matbouli M, Saleh M. Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine. Animals. 2023;13(7):1250. doi:10.3390/ani13071250
  18. Wang Y, Cui X, Chen Z. Innovations in cattle breeding technology: Prospects in the era of gene editing. Animals. 2025;15(10):1364.
  19. Singh P, Ali SA. Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals. Veterinary Sciences. 2021;8(7):122. doi:10.3390/vetsci8070122
  20. Hansen PJ. Reproductive physiology of the heat-stressed dairy cow: implications for fertility and assisted reproduction. Animal Reproduction. 2019;16(3):497-507. doi:10.21451/1984-3143-ar2019-0053
  21. Islam MA, Rony SA, Rahman MB, Cinar MU, Villena J, Uddin MJ, et al. Improvement of Disease Resistance in Livestock: Application of Immunogenomics and CRISPR/Cas9 Technology. Animals. 2020;10(12):2236. doi:10.3390/ani10122236
  22. Tizard M, Hallerman E, Fahrenkrug S, Newell-McGloughlin M, Gibson J, de Loos F, et al. Strategies to enable the adoption of animal biotechnology to sustainably improve global food safety and security. Transgenic Research. 2016;25(5):575-595. doi:10.1007/s11248-016-9965-1
  23. von Keyserlingk MAG, Mills KE, Weary DM. Attitudes of western Canadian dairy farmers toward technology. Journal of Dairy Science. 2024;107(2):933-943. doi:10.3168/jds.2023-23279
  24. Gatica-Arias A. The regulatory current status of plant breeding technologies in some Latin American and the Caribbean countries. Plant Cell, Tissue and Organ Culture (PCTOC). 2020;141(2):229-242. doi:10.1007/s11240-020-01799-1
  25. Wadood AA, Bordbar F, Zhang X. Integrating omics approaches in livestock biotechnology: innovations in production and reproductive efficiency. Frontiers in Animal Science. 2025;6. doi:10.3389/fanim.2025.1551244
  26. Van Eenennaam AL, De Figueiredo Silva F, Trott JF, Zilberman D. Genetic Engineering of Livestock: The Opportunity Cost of Regulatory Delay. Annual Review of Animal Biosciences. 2021;9(1):453-478. doi:10.1146/annurev-animal-061220-023052
  27. Wolabu TW, Mahmood K, Chen F, Torres-Jerez I, Udvardi M, Tadege M, et al. Mutating alfalfa COUMARATE 3-HYDROXYLASE using multiplex CRISPR/Cas9 leads to reduced lignin deposition and improved forage quality. Frontiers in Plant Science. 2024;15. doi:10.3389/fpls.2024.1363182
  28. Huang L, Zhou M, Abbas G, Li C, Cui M, Zhang XE, et al. A Cancer Cell Membrane‐Derived Biomimetic Nanocarrier for Synergistic Photothermal/Gene Therapy by Efficient Delivery of CRISPR/Cas9 and Gold Nanorods. Advanced Healthcare Materials. 2022;11(16). doi:10.1002/adhm.202201038
  29. Shriver A, McConnachie E. Genetically Modifying Livestock for Improved Welfare: A Path Forward. Journal of Agricultural and Environmental Ethics. 2018;31(2):161-180. doi:10.1007/s10806-018-9719-6
  30. Qazi S, Miettinen HM, Wilkinson RA, McCoy K, Douglas T, Wiedenheft B. Programmed Self-Assembly of an Active P22-Cas9 Nanocarrier System. Molecular Pharmaceutics. 2016;13(3):1191-1196. doi:10.1021/acs.molpharmaceut.5b00822
  31. Yang B. Enhancing Dairy Cow Milk Fat Synthesis Genes with CRISPRCas9 Technology to Increase Dairy Product Yield. International Journal of Molecular Veterinary Research. 2024. doi:10.5376/ijmvr.2024.14.0002
  32. Martin P, Grosclaude F. Improvement of milk protein quality by gene technology. Livestock Production Science. 1993;35(1-2):95-115. doi:10.1016/0301-6226(93)90184-j
  33. Priyadharsini R, Jayalalitha V, Arulkumar S. Designer milk: In the view of molecular genetics. Int J Vet Sci Anim Husb. 2023;65–72. Available from: www.veterinarypaper.com
  34. Thompson-Crispi K, Atalla H, Miglior F, Mallard BA. Bovine Mastitis: Frontiers in Immunogenetics. Frontiers in Immunology. 2014;5. doi:10.3389/fimmu.2014.00493
  35. Hou S, Wang X, Ren S, Meng X, Yin X, Zhang J, et al. Knockout of HDAC9 Gene Enhances Foot-and-Mouth Disease Virus Replication. Frontiers in Microbiology. 2022;13. doi:10.3389/fmicb.2022.805606
  36. Mor A, Mondal S, Reddy IJ, Soumya NP. Genes Regulating Maternal Recognition of Pregnancy in Domestic Animals: an Update. Brazilian Archives of Biology and Technology. 2015;58(6):854-863. doi:10.1590/s1516-89132015060284
  37. Lefebvre R, Larroque H, Barbey S, Gallard Y, Colleau JJ, Lainé AL, et al. Genome-wide association study for age at puberty and resumption of cyclicity in a crossbred dairy cattle population. Journal of Dairy Science. 2021;104(5):5794-5804. doi:10.3168/jds.2020-18228
  38. Brito LF, Oliveira HR, Houlahan K, Fonseca PAS, Lam S, Butty AM, et al. Genetic mechanisms underlying feed utilization and implementation of genomic selection for improved feed efficiency in dairy cattle. Canadian Journal of Animal Science. 2020;100(4):587-604. doi:10.1139/cjas-2019-0193
  39. Beever DE, Doyle PT. Feed conversion efficiency as a key determinant of dairy herd performance: a review. Australian Journal of Experimental Agriculture. 2007;47(6):645-657. doi:10.1071/ea06048
  40. Baldwin RL, Smith NE, Taylor J, Sharp M. Manipulating Metabolic Parameters to Improve Growth Rate and Milk Secretion. Journal of Animal Science. 1980;51(6):1416-1428. doi:10.2527/jas1981.5161416x
  41. Worku D, Hussen J, De Matteis G, Schusser B, Alhussien MN. Candidate genes associated with heat stress and breeding strategies to relieve its effects in dairy cattle: a deeper insight into the genetic architecture and immune response to heat stress. Frontiers in Veterinary Science. 2023;10. doi:10.3389/fvets.2023.1151241
  42. Polsky L, von Keyserlingk MAG. Invited review: Effects of heat stress on dairy cattle welfare. Journal of Dairy Science. 2017;100(11):8645-8657. doi:10.3168/jds.2017-12651
  43. Rovelli G, Ceccobelli S, Perini F, Demir E, Mastrangelo S, Conte G, et al. The genetics of phenotypic plasticity in livestock in the era of climate change: a review. Italian Journal of Animal Science. 2020;19(1):997-1014. doi:10.1080/1828051x.2020.1809540
  44. Løvendahl P, Difford GF, Li B, Chagunda MGG, Huhtanen P, Lidauer MH, et al. Review: Selecting for improved feed efficiency and reduced methane emissions in dairy cattle. Animal. 2018;12:s336-s349. doi:10.1017/s1751731118002276
  45. Abd El-Hack ME, Abdelnour SA, Kamal M, Khafaga AF, Shakoori AM, Bagadood RM, et al. RETRACTED: Lactoferrin: Antimicrobial impacts, genomic guardian, therapeutic uses and clinical significance for humans and animals. Biomedicine & Pharmacotherapy. 2023;164:114967. doi:10.1016/j.biopha.2023.114967
  46. Nguyen QV, Malau-Aduli BS, Cavalieri J, Nichols PD, Malau-Aduli AEO. Enhancing Omega-3 Long-Chain Polyunsaturated Fatty Acid Content of Dairy-Derived Foods for Human Consumption. Nutrients. 2019;11(4):743. doi:10.3390/nu11040743
  47. Calder PC. Omega-3 fatty acids and inflammatory processes: from molecules to man. Biochemical Society Transactions. 2017;45(5):1105-1115. doi:10.1042/bst20160474
  48. Gerardi AS. Bovine leucocyte adhesion deficiency: A review of a modern disease and its implications. Research in Veterinary Science. 1996;61(3):183-186. doi:10.1016/s0034-5288(96)90060-0
  49. Koltes JE, Mishra BP, Kumar D, Kataria RS, Totir LR, Fernando RL, et al. A nonsense mutation in cGMP-dependent type II protein kinase ( PRKG2 ) causes dwarfism in American Angus cattle. Proceedings of the National Academy of Sciences. 2009;106(46):19250-19255. doi:10.1073/pnas.0904513106
  50. Sonstegard TS, Flórez JM, Garcia JF. Commercial perspectives: Genome editing as a breeding tool for health and well-being in dairy cattle. JDS Communications. 2024;5(6):767-771. doi:10.3168/jdsc.2023-0481
  51. Wang M, Ibeagha-Awemu EM. Impacts of Epigenetic Processes on the Health and Productivity of Livestock. Frontiers in Genetics. 2021;11. doi:10.3389/fgene.2020.613636
  52. Prayaga KC. Genetic options to replace dehorning in beef cattle—a review*. Australian Journal of Agricultural Research. 2007;58(1):1-8. doi:10.1071/ar06044
  53. Ariyarathne HBPC, Correa-Luna M, Blair H, Garrick D, Lopez-Villalobos N. Can Nitrogen Excretion of Dairy Cows Be Reduced by Genetic Selection for Low Milk Urea Nitrogen Concentration? Animals. 2021;11(3):737. doi:10.3390/ani11030737
  54. Plaizier JC, Danesh Mesgaran M, Derakhshani H, Golder H, Khafipour E, Kleen JL, et al. Review: Enhancing gastrointestinal health in dairy cows. Animal. 2018;12:s399-s418. doi:10.1017/s1751731118001921
  55. Pal A, Chakravarty AK. Disease resistance for different livestock species. Genetics and Breeding for Disease Resistance of Livestock. 2020:271-296. doi:10.1016/b978-0-12-816406-8.00019-x
  56. Suva LJ, Westhusin ME, Long CR, Gaddy D. Engineering bone phenotypes in domestic animals: Unique resources for enhancing musculoskeletal research. Bone. 2020;130:115119. doi:10.1016/j.bone.2019.115119
  57. Mohammadabadi M, Bordbar F, Jensen J, Du M, Guo W. Key Genes Regulating Skeletal Muscle Development and Growth in Farm Animals. Animals. 2021;11(3):835. doi:10.3390/ani11030835
  58. Laible G, Wei J, Wagner S. Improving livestock for agriculture – technological progress from random transgenesis to precision genome editing heralds a new era. Biotechnology Journal. 2014;10(1):109-120. doi:10.1002/biot.201400193
  59. BHARTI VK, GIRI A, VIVEK P, KALIA S. Health and productivity of dairy cattle in high altitude cold desert environment of Leh-Ladakh: A review. The Indian Journal of Animal Sciences. 2017;87(1). doi:10.56093/ijans.v87i1.66794
  60. Whitelaw CBA, Joshi A, Kumar S, Lillico SG, Proudfoot C. Genetically engineering milk. Journal of Dairy Research. 2016;83(1):3-11. doi:10.1017/s0022029916000017
  61. Hay AN, Farrell K, Leeth CM, Lee K. Use of Genome Editing Techniques to Produce Transgenic Farm Animals. Advances in Experimental Medicine and Biology. 2021:279-297. doi:10.1007/978-3-030-85686-1_14
  62. Clark EL. Breeding in an Era of Genome Editing. Encyclopedia of Sustainability Science and Technology. 2022:1-16. doi:10.1007/978-1-4939-2493-6_1122-1
  63. Liu Z, Wu T, Xiang G, Wang H, Wang B, Feng Z, et al. Enhancing Animal Disease Resistance, Production Efficiency, and Welfare through Precise Genome Editing. International Journal of Molecular Sciences. 2022;23(13):7331. doi:10.3390/ijms23137331
  64. Raza SHA, Hassanin AA, Pant SD, Bing S, Sitohy MZ, Abdelnour SA, et al. Potentials, prospects and applications of genome editing technologies in livestock production. Saudi Journal of Biological Sciences. 2022;29(4):1928-1935. doi:10.1016/j.sjbs.2021.11.037
  65. Long M, Wang B, Yang Z, Lu X. Genome-Wide Association Study as an Efficacious Approach to Discover Candidate Genes Associated with Body Linear Type Traits in Dairy Cattle. Animals. 2024;14(15):2181. doi:10.3390/ani14152181
  66. Buaban S, Lengnudum K, Boonkum W, Phakdeedindan P. Genome-wide association study on milk production and somatic cell score for Thai dairy cattle using weighted single-step approach with random regression test-day model. Journal of Dairy Science. 2022;105(1):468-494. doi:10.3168/jds.2020-19826
  67. Cai W, Li C, Liu S, Zhou C, Yin H, Song J, et al. Genome Wide Identification of Novel Long Non-coding RNAs and Their Potential Associations With Milk Proteins in Chinese Holstein Cows. Frontiers in Genetics. 2018;9. doi:10.3389/fgene.2018.00281
  68. Haskell MJ, Simm G, Turner SP. Genetic selection for temperament traits in dairy and beef cattle. Frontiers in Genetics. 2014;5. doi:10.3389/fgene.2014.00368
  69. Rørvang MV, Nielsen BL, Herskin MS, Jensen MB. Prepartum Maternal Behavior of Domesticated Cattle: A Comparison with Managed, Feral, and Wild Ungulates. Frontiers in Veterinary Science. 2018;5. doi:10.3389/fvets.2018.00045
  70. Milanesi M, Passamonti MM, Cappelli K, Minuti A, Palombo V, Sgorlon S, et al. Genetic Regulation of Biomarkers as Stress Proxies in Dairy Cows. Genes. 2021;12(4):534. doi:10.3390/genes12040534
  71. Wang X, Guo L, Zhang W. Extraction of Innate Immune Genes in Dairy Cattle and the Regulation of Their Expression in Early Embryos. Genes. 2024;15(3):372. doi:10.3390/genes15030372
  72. Quinzo MJ, Perteguer MJ, Brindley PJ, Loukas A, Sotillo J. Transgenesis in parasitic helminths: a brief history and prospects for the future. Parasites & Vectors. 2022;15(1). doi:10.1186/s13071-022-05211-z
  73. Ajaz M, Rasool W, Mahmood A. Comprehensive Review of Nanotechnology: Innovations and Multidisciplinary Applications. Futuristic Biotechnology. 2024:12-18. doi:10.54393/fbt.v4i01.81
  74. Ramani A, Taherabbas S, Saji R, Bumbadiya M, Gandhi K, Seth R. Nanotechnology: An emerging trend in the dairy industry – Applications and future challenges. Food and Humanity. 2024;3:100409. doi:10.1016/j.foohum.2024.100409
  75. El-Nile AE, Elazab MA, Soltan YA, Elkomy AE, El-Zaiat HM, Sallam SMA, et al. Nano and natural zeolite feed supplements for dairy goats: Feed intake, ruminal fermentation, blood metabolites, and milk yield and fatty acids profile. Animal Feed Science and Technology. 2023;295:115522. doi:10.1016/j.anifeedsci.2022.115522
  76. Liu Y, Zhang J, Bu L, Huo W, Pei C, Liu Q. Effects of nanoselenium supplementation on lactation performance, nutrient digestion and mammary gland development in dairy cows. Animal Biotechnology. 2023;35(1). doi:10.1080/10495398.2023.2290526