2 publications
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Published Subscription
Isolation and Characterization of “Heavy Chain Antibody” Variable Domain Gene of Indian Desert Camel (Camelus dromedarius, L.)By Ajit Singh, Neelam Pandey
Abstract: Camelids have “heavy chain antibodies” (HCAbs) of IgG isotype (IgG2 and IgG3 subtypes) and lack light chains and heavy chain constant domain 1 (CH1) in their structure. The molecular biological features of VHH (i.e., antigen binding variable domain of HCAbs) gene isolated from Indian desert camel were examined as a prelude to construct VHHs-phage display library. VHH cDNA was synthesized by reverse transcription-polymerase chain reaction (RT-PCR) from B lymphocyte mRNA, …
Published in Research and Reviews : A Journal of Immunology · Vol. 3, Issue 1, 2013 · pp. 22–31 Read article →
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Published Subscription
Production of Single-domain Antibodies Library by Phage Display TechnologyBy Ajit Singh, Neelam Pandey, Pooja Jangra, Jagveer Rawat
Abstract: Single-domain antibodies (dAbs) are synthesized from variable domain gene-segments of atypical camelid or shark “heavy chain antibodies” (HCAbs) by phage display technology. We have constructed a phage display library of > 5 × 107 dAb clones derived from HCAb genes in B lymphocyte repertoire of a lipopolysaccharide (LPS)-immunized Indian desert camel and isolated dAb-phage clones by panning of the library against LPS and Staphylococcus aureus exotoxins. Nucleotide and deduced amino-acid …
Published in Research and Reviews : A Journal of Immunology · Vol. 2, Issue 3, 2012 · pp. 8–13 Read article →