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• Published Subscription 2 Apr 2019

  Production of Single-domain Antibodies Library by Phage Display Technology

  By Ajit Singh, Neelam Pandey, Pooja Jangra, Jagveer Rawat

  Abstract: Single-domain antibodies (dAbs) are synthesized from variable domain gene-segments of atypical camelid or shark “heavy chain antibodies” (HCAbs) by phage display technology. We have constructed a phage display library of > 5 × 107 dAb clones derived from HCAb genes in B lymphocyte repertoire of a lipopolysaccharide (LPS)-immunized Indian desert camel and isolated dAb-phage clones by panning of the library against LPS and Staphylococcus aureus exotoxins. Nucleotide and deduced amino-acid …

  Published in Research and Reviews : A Journal of Immunology · Vol. 2, Issue 3, 2012 · pp. 8–13 Read article →