STM Journals Peer-reviewed research from Consortium e-Learning Network

7 publications

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    Effect of the Length of Antisense RNA on Bacterial Enzyme Production

    By Gerald Goldstein, E. T. McKenzie, N. R. Reed, A. C. Smith

    Abstract: Normal 0 false false false EN-US X-NONE X-NONE Antisense RNA molecules for the beta-galactosidase enzyme of E. coli and the urease enzyme of Proteus vulgaris were synthesized using the polymerase chain reaction. The artificial genes were designed to produce single stranded RNA molecules complementary to various lengths of the 5’ end of the messenger RNA molecules of the two bacterial enzymes. Four different antisense RNA molecules for each enzyme were …

    Published in Research and Reviews : A Journal of Biotechnology · Vol. 7, Issue 2, 2017 · pp. 19–24 Read article

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    Turmeric Extract and Curcumin Enhance the Yield of T2 Bacteriophage in E. coli

    By Gerald Goldstein, Sarah M. Bergman, Mary A. Cranley, Nicholas R. Reed

    Abstract: Normal 0 false false false EN-US X-NONE X-NONE It has been demonstrated that turmeric and curcumin—a chemical present in turmeric—have antibacterial and antiviral activity for viruses of humans. Increasing concentrations of turmeric extract and purified curcumin have no effect on the replication of Escherichia coli cultured in Luria-Bertani broth. Increasing concentrations of curcumin enhance the replication of T2 bacteriophage in E. coli cells. E. coli cells treated with purified curcumin …

    Published in Research and Reviews : A Journal of Biotechnology · Vol. 6, Issue 3, 2016 · pp. 19–26 Read article

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    Differential Susceptibility of Bacteriophage and Viruses to Reactive Oxygen Species

    By Gerald Goldstein

    Abstract: Normal 0 false false false EN-US X-NONE X-NONE Reactive oxygen species of superoxide, hydrogen peroxide, and hydroxyl radicals were generated by mixing xanthine, xanthine oxidase and iron citrate and incubating for 5 min at room temperature. T2 bacteriophage, encephalomyocarditis (EMC) virus, adenovirus, or vaccinia virus particles were incubated with the solution for various periods of time after reactive oxygen species were formed. Physical damage to the virus particles that affected …

    Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 6, Issue 2, 2016 · pp. 7–12 Read article

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    Comparison of the Keratinase Gene Sequences of Fast, Slow and Intermediate Feather Degrading Strains of Bacillus licheniformis

    By Gerald Goldstein, Patricia BS Celestino-Soper, Allison K Morrell Dailey, Sara Fitzgerald-Butt

    Abstract: Feathers are composed of beta keratin which is an insoluble protein resistant to degradation. Bacillus licheniformis has a gene that codes for a keratinase enzyme that hydrolyzes feathers. The DNA sequences of the genes coding for keratinase enzymes of various strains of B. licheniformis were compared. Some strains of the bacteria hydrolyze feathers rapidly, some slowly, and some at an intermediate rate. The mature keratinase enzyme is composed of 274 …

    Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 3, 2015 · pp. 1–9 Read article

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    Sodium Fluoride Enhances T2 Bacteriophage Yield

    By Gerald Goldstein, J. M. Quinn

    Abstract: Sodium Fluoride is a strong stimulator of the adenylate cyclase enzyme both in eucaryotic and in procaryotic cells. Stimulation of adenylate cyclase produces cAMP and increases the metabolic activity of bacterial cells by methods that are as yet not completely understood. The addition of either sodium chloride or sodium bromide to bacteriological growth medium moderately affects the growth rate of E. coli cells after 2 h of exposure while the …

    Published in Research and Reviews : A Journal of Biotechnology · Vol. 5, Issue 2, 2015 · pp. 17–23 Read article

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    Garlic and Onion Extracts Enhance the Yield of T2 Bacteriophage in E. coli

    By Gerald Goldstein, Owen L. Kelling, Gregory L. Serpa, Karli L. Sturgill, Madeleine M. Vroom

    Abstract: It has been demonstrated that garlic and some of its components have anti-bacterial and/or anti-viral activity for viruses of humans in vitro. Increasing concentrations of garlic extract have no effect on the replication of E. coli cultured in nutrient broth. Increasing concentrations of garlic extract inhibit the replication of T4 Bacteriophage in E. coli but, surprisingly, E. coli cells treated with garlic extract produced a yield of T2 Bacteriophage up …

    Published in Research and Reviews : A Journal of Biotechnology · Vol. 5, Issue 2, 2015 · pp. 8–16 Read article

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    Inhibition of Replication of T2 Bacteriophage in Escherichia coli by 6-diazo-5-oxo-L-norleucine (DON)

    By Gerald Goldstein, Amanda R. Robinson, Christina L. Vorobej, Umut Aypar, Heather M. Costello

    Abstract: 800x600 Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 The antibiotic known as 6-diazo-5-oxo-L-norleucine, DON, inhibits the growth of several types of Gram positive and Gram negative bacteria. It has also been demonstrated that DON inhibits the replication of poliovirus, respiratory syncytial virus, mumps and herpes simplex virus in cultured eukaryotic cells. At a concentration of 0.03 mM DON, the replication of Escherichia coli cells was inhibited by 11.0% …

    Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 2, Issue 1, 2012 Read article

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